[EMBOSS] Genpept entry in MSE

[Steve Taylor]

Hi,

I am trying to load a Genpept entry into MSE, EMBOSS Version 6.0.1 on Fedora. Unfortunately it doesn't like the LOCUS line.

It loads, but warns:

Warning: bad Genbank LOCUS line 'LOCUS       ACN78416                 225 aa          linear   BCT 21-MAR-2009'

Changing the aa to bp fixes it.

Thanks,

Steve


LOCUS       ACN78416                 225 aa            linear   BCT 21-MAR-2009
DEFINITION  galactosyltransferase A [Pasteurella multocida].
ACCESSION   ACN78416
VERSION     ACN78416.1  GI:224999306
DBSOURCE    accession FJ755839.1
KEYWORDS    .
SOURCE      Pasteurella multocida
  ORGANISM  Pasteurella multocida
            Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Pasteurella.
REFERENCE   1  (residues 1 to 225)
  AUTHORS   Boyce,J.D., Harper,M., St Michael,F., John,M., Aubry,A., Parnas,H.,
            Logan,S.M., Wilkie,I.W., Ford,M., Cox,A.D. and Adler,B.
  TITLE     Identification of novel glycosyltransferases required for assembly
            of the Pasteurella multocida A:1 lipopolysaccharide and their
            involvement in virulence
  JOURNAL   Infect. Immun. 77 (4), 1532-1542 (2009)
   PUBMED   19168738
REFERENCE   2  (residues 1 to 225)
  AUTHORS   Harper,M., John,M., Adler,B. and Boyce,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-FEB-2009) Microbiology, Monash University, Wellington
            road, Clayton, Melbourne, Victoria 3800, Australia
COMMENT     Method: conceptual translation supplied by author.
FEATURES             Location/Qualifiers
     source          1..225
                     /organism="Pasteurella multocida"
                     /strain="VP161"
                     /serotype="1"
                     /db_xref="taxon:747"
                     /note="serogroup: A"
     Protein         1..225
                     /product="galactosyltransferase A"
                     /function="adds beta-D-galactose to both the 4 and 6
                     position of alpha-L,D-heptose IV"
                     /name="putative bi-functional galactosyltransferase; GatA"
     Region          5..188
                     /region_name="Glyco_transf_25"
                     /note="Glycosyltransferase family 25 [lipooligosaccharide
                     (LOS) biosynthesis protein] is a family of
                     glycosyltransferases involved in LOS biosynthesis. The
                     members include the beta(1,4) galactosyltransferases: Lgt2
                     of Moraxella catarrhalis, LgtB and LgtE of...; cd06532"
                     /db_xref="CDD:133474"
     CDS             1..225
                     /gene="gatA"
                     /coded_by="FJ755839.1:1..678"
                     /transl_table=11
ORIGIN      
        1 mklpkiivis lknsprrqii shrlsglgld feffdavygk dltkeeleki dyeffpkycg
       61 skgaltlgei gcamshikiy ehivannleq viileddaiv slyfeeivla alqklpnrre
      121 ilfldhgkak vypfmrnlpe ryrlaryrkp skhskrfivr ttaylitleg akkllkhayp
      181 irmpsdfltg llqlthinay giepscvfgg veseinemer raglk
//