[BioRuby] Melting Temperature

[Naohisa Goto]

Hi,

I've found another implementation to calculate Tm in a blog:
http://d.hatena.ne.jp/fukuit/20101022/1287765340
Although the blog is written in Japanese, the Ruby code is clear.
The code resembles with Bioroebe's code because the same formula
is used to calculate Tm.

Naohisa Goto
ngoto at gen-info.osaka-u.ac.jp / ng at bioruby.org

> Hi David
> 
> The 'bioroebe' gem includes Tm calculation.
> 
>     $ gem install bioroebe
>     $ irb
> 
>     > require 'bioroebe/calculate_melting_temperature'
>     > seq = 'ATGCTGACGTATGCGATCGTA'
>     > tm = CalculateMeltingTemperature.new
>     > tm.set_input(seq)
>     > tm.calculate_tm
>     # ==> 65.32166985617363
> 
> You can set oligo nucleotide concentration, Na+ conc and
> formamide_concentration.
> 
> The method isn't too complicated
> <http://rubydoc.info/gems/bioroebe/0.0.44/CalculateMeltingTemperature:calculate_tm>
> 
> Rob Syme
> 
> On Thu, Oct 9, 2014 at 6:10 AM, McCreavy David <David.McCreavy at lhch.nhs.uk>
> wrote:
> 
> > Hi Guys,
> >
> > Where in the API will I find methods for calculating Tm?
> >
> > Kind Regards, David
> >
> >
> >
> >
> >
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