[BioRuby] Melting Temperature

Rob Syme rob.syme at gmail.com
Thu Oct 9 14:35:34 UTC 2014


Hi David

The 'bioroebe' gem includes Tm calculation.

    $ gem install bioroebe
    $ irb

    > require 'bioroebe/calculate_melting_temperature'
    > seq = 'ATGCTGACGTATGCGATCGTA'
    > tm = CalculateMeltingTemperature.new
    > tm.set_input(seq)
    > tm.calculate_tm
    # ==> 65.32166985617363

You can set oligo nucleotide concentration, Na+ conc and
formamide_concentration.

The method isn't too complicated
<http://rubydoc.info/gems/bioroebe/0.0.44/CalculateMeltingTemperature:calculate_tm>

Rob Syme

On Thu, Oct 9, 2014 at 6:10 AM, McCreavy David <David.McCreavy at lhch.nhs.uk>
wrote:

> Hi Guys,
>
> Where in the API will I find methods for calculating Tm?
>
> Kind Regards, David
>
>
>
>
>
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