[Biopython] running BLAST remotely and restricting search to a specific organism's wgs database

Chrysoula Pantzartzi chrysoula.pantzartzi at img.cas.cz
Tue Nov 3 14:31:40 UTC 2015


>> Dear all
>>
>> I am really new in programming and python. I am trying to run BLAST 
>> remotely, using the Biopython NCBIWWW module. I want to search 
>> against the wgs database for different species and each time restrict 
>> the search to a specific organism's wgs database. When I BLAST vs the 
>> nr database, I use the entrez_query=species[Organism], as suggested 
>> in the NCBI webpage and the biopython webpage and forums. 
>> Unfortunately, this does not work with wgs subdivision. In fact, 
>> entrez query is not available on the online BLAST page when wgs is 
>> chosen as the database - only limit by organism etc etc. I even tried 
>> the limit_by argument, but I get an error, that this is not an 
>> argument in the NCBIWWW module.
>>
>>  Could you please advise me on the best solution?
>>
>> Thank you in advance
>> Chrysa
>



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