[Biopython-dev] sff reader

Peter biopython at maubp.freeserve.co.uk
Thu Aug 13 13:38:43 EDT 2009


Peter wrote:
>
> Sadly the format of the index isn't documented, but I think I have
> reverse engineered the format that Roche SFF files are using. In a
> slight twist of the specification they are actually using the index bock
> for both XML meta data AND and index of the read offsets.

I'm not the first to notice this, see for example the Celera Assembler
looks in a Roche SFF file's XML meta data to determine how the
quality scores were called:

http://sourceforge.net/apps/mediawiki/wgs-assembler/index.php?title=Roche_454_Platforms

Peter


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