[Bioperl-l] Bio::Tools::Run::RemoteBlast: RID not found

Mon Aug 11 23:32:52 EDT 2003

Dear All

this is very likely a trivial question:

I am using the Bio::Tools::Run::RemoteBlast module to run blast and 
every other request bounces back with the following error:

-------------------- WARNING ---------------------
MSG:

<p><!--
QBlastInfoBegin
         Status=UNKNOWN
QBlastInfoEnd
--><p><BODY BGCOLOR="#FFFFFF">
<hr><font color="red">ERROR: Results for RID 1060657821-20728-1846636 
not found</font><hr>

my code is a sad copy of the bptutorial (see below): I would really 
appreciate some help,

Eric

....
$database =  'nr';
@params = ('-prog'   => 'blastn',
              '-data'   => $database);
$Bio::Tools::Run::RemoteBlast::HEADER{'WORD SIZE'} = '7';
$Bio::Tools::Run::RemoteBlast::HEADER{'FILTER'} = 'OFF';
$Bio::Tools::Run::RemoteBlast::HEADER{'EXPECT'} = '1000';
	$remote_blast_object = Bio::Tools::Run::RemoteBlast->new(@params);
	$r = $remote_blast_object->submit_blast($seq1);
	while ( my @rids = $remote_blast_object->each_rid ) {
	foreach my $rid ( @rids ) {
		sleep 20;
		$rc = $remote_blast_object->retrieve_blast($rid);
		if( !ref($rc) ) {   # $rc not a reference => either error
		  # or job not yet finished
		  if( $rc < 0 ) {
			  $remote_blast_object->remove_rid($rid);
			  print"Error return code for BlastID code $rid ... \n";
		  }
		  sleep 5;
		} else {
		  $remote_blast_object->remove_rid($rid);
...