[Biojava-dev] Reg: Quality values of ABI File

Sreekanth Mogullapally sreekanth.m at ocimumbio.com
Mon Jul 13 14:49:26 UTC 2009


Dear Franklin,

Thank you very much for your quick responce.
Now I can able to get the Quality values, which meets my requirement.


Thanks & Regards,
Sreekanth.M

From: Franklin Bristow [mailto:fbristow at gmail.com]
Sent: Monday, July 13, 2009 6:46 PM
To: Sreekanth Mogullapally
Cc: biojava-dev-request at lists.open-bio.org; biojava-dev at lists.open-bio.org; Madhu Mohan. Ganni; Kishore Dunga
Subject: Re: [Biojava-dev] Reg: Quality values of ABI File

Hi Sreekanth,
The quality values are stored under the PCON 1 and 2 tags.  The information you need is in the offsetData field of the TaggedDataRecord.  You can treat this byte array as an array of shorts containing the quality values for each base.

Take a look at this PDF for more information about the different tags available to you:
http://www.appliedbiosystems.com/support/software_community/ABIF_File_Format.pdf

--
Franklin
On Mon, Jul 13, 2009 at 6:28 AM, Sreekanth Mogullapally <sreekanth.m at ocimumbio.com<mailto:sreekanth.m at ocimumbio.com>> wrote:
Hi Everybody,

I am working with "ABI" Files. I am able to get the pixel values for Chromatogram viewer from it,
but I need the Quality values for each base.
Please help me in this regard.

Thanks in Advance
Sreekanth.M


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