[BioPython] check for primer in primer3 or biopython?

[Andrew Nunberg]

Primer3 does that.  You can set parameters for how much primer-overlap, %GC,
etc...

-- 
Andrew Nunberg
Bioinformagician
Orion Genomics
(314)-615-6989
www.oriongenomics.com


> From: Sebastian Bassi <sbassi at asalup.org>
> Organization: ASALUP
> Reply-To: sbassi at asalup.org
> Date: Wed, 15 Oct 2003 10:21:46 -0300
> To: biopython at biopython.org
> Subject: [BioPython] check for primer in primer3 or biopython?
> 
> Hello,
> 
> Is there a way to check if a specific primer is good for a PCR reation?
> Primer3 can pick primers given a target sequence, but what I want is to
> test how "good" is a specific primer pair that was choosen previously
> (by hand or by another program).
> By good I mean that both primers wont anneal each other, they won't
> anneal itself, stability, missmatch percent, and so on.
> 
> -- 
> Best regards,
> 
> //=\ Sebastian Bassi - Diplomado en Ciencia y Tecnologia, UNQ   //=\
> \=// IT Manager Advanta Seeds - Balcarce Research Center -      \=//
> //=\ Pro secretario ASALUP - www.asalup.org - PGP key available //=\
> \=// E-mail: sbassi at genesdigitales.com - ICQ UIN: 3356556 -     \=//
> 
>                http://Bioinformatica.info
> 
> 
> 
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