[BioPython] check for primer in primer3 or biopython?

Sebastian Bassi sbassi at asalup.org
Wed Oct 15 09:21:46 EDT 2003


Hello,

Is there a way to check if a specific primer is good for a PCR reation? 
Primer3 can pick primers given a target sequence, but what I want is to 
test how "good" is a specific primer pair that was choosen previously 
(by hand or by another program).
By good I mean that both primers wont anneal each other, they won't 
anneal itself, stability, missmatch percent, and so on.

-- 
Best regards,

//=\ Sebastian Bassi - Diplomado en Ciencia y Tecnologia, UNQ   //=\
\=// IT Manager Advanta Seeds - Balcarce Research Center -      \=//
//=\ Pro secretario ASALUP - www.asalup.org - PGP key available //=\
\=// E-mail: sbassi at genesdigitales.com - ICQ UIN: 3356556 -     \=//

                 http://Bioinformatica.info





More information about the BioPython mailing list