[EMBOSS] vectorstrip on FASTQ files

Peter biopython at maubp.freeserve.co.uk
Wed Aug 19 07:31:17 EDT 2009


 Peter Rice wrote:
>
> Peter C. wrote:
>> Hi,
>>
>> I'm trying to use vectorstrip on FASTQ files (as a simple way to
>> remove adaptor or primer sequences). However, it seems that on output
>> the FASTQ qualities are missing (all set to the double quote, ASCII
>> 33, meaning PHRED quality 1 or random). Is this a known bug (or
>> rather, a missing feature)?
>
> It is a missing feature. vectorstrip was written before quality scores
> became fashionable and, curiously, nobody has asked for them before.
>
> We will certainly retain them in a future release.

Great - thanks!

Peter C.


More information about the EMBOSS mailing list