[emboss-dev] EMBOSS seqret FASTQ support

[Peter]

On Fri, Jul 24, 2009 at 11:32 AM, Peter<biopython at maubp.freeserve.co.uk> wrote:
>
> Have I understood correctly? i.e. in EMBOSS 6.1.0:
>
> "fastq" on input - ignores quality strings
> "fastq" on output - acts like "fastq-sanger"
> "fastq-sanger" - PHRED scores offset 31 [* TYPO - should be offset 33 *]
> "fastq-solexa" - Solexa scores offset 64
> "fastq-illumina" - PHRED scores offset 64
>

Correction (just for the record) - the Sanger FASTQ files use
an offset of 33 (ASCII for "!"). The number 31 is important as
the difference between this and the Illumina ASCII offset.

Peter C.