[BioRuby] FastQ and Quality Scores

Raoul Bonnal bonnal at ingm.org
Sun Mar 27 17:11:53 UTC 2011


Thanks Peter,
seqanswer  very useful.
I still have concerns about loading by default in sanger, at least now ... btw in the near future it will re-became de facto standard. Confusion is generated from companies obviously.
The discussion in the article is still valid if we must live in a mixed world -_-'

On 27/mar/2011, at 00.17, Peter Cock wrote:

> On Fri, Mar 25, 2011 at 5:06 PM, Raoul Bonnal <bonnal at ingm.org> wrote:
>> Dear All,
>> probably I don't know very much this part of the bioruby library (not yet).
>> In this days I'm playing a bit with fastq data :-) and bioruby
>> ...
>> 
>> Is Illumina the right format for the newest technology/datasets ?
> 
> Right now, yes, but with the imminent release of Illumina's
> CASAVA 1.8 pipeline, they will start using the standard
> Sanger FASTQ encoding.
> http://seqanswers.com/forums/showthread.php?t=8895
> 
>> ...
>> I'm lost O_o
> 
> Hopefully this will help:
> http://dx.doi.org/10.1093/nar/gkp1137
> 
> Peter
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Ra

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