[Biopython] going from protein to gene to oligos for cloning

[David Shin]

Hi again,

I'm trying to use biopython to help me grab a lot of protein sequences that
will eventually be used as the basis for cloning. I'm almost done screening
my protein sequences, and pretty much ok on that part...

I was just curious if anyone has already developed, or has any decent
advice on going from protein codes to getting the actual coding sequences
of the genes.

At this point, my plan is to take protein codes (ie. numbers in
gi|145323746|) and use these to search entrez nucleotide databases directly
to get hits (I have tested it once seems to work to get genbank records...
then try to use the information inside to get the nucleotide sequences...
or I guess the other way is to use the top hit from tblastn somehow?

Thanks,

Dave



-- 
David Shin, Ph.D
Lawrence Berkeley National Labs
1 Cyclotron Road
MS 83-R0101
Berkeley, CA 94720
USA