[Biopython] Paired-End Read Splitting & Joining

Peter Cock p.j.a.cock at googlemail.com
Thu Nov 17 14:28:03 UTC 2011


On Thu, Nov 17, 2011 at 1:39 PM, Fields, Christopher J
<cjfields at illinois.edu> wrote:
>
> This is one of my gripes about the SRA tools, that they (by default)
> dump paired-end data as one concatenated string; it's a nasty gotcha.
> You need to specify the --split-files option to fastq-dump to dump
> these as paired end, and this will split them into two files.

Thank Chris - that was one of the possibilities I wanted to check,
but couldn't see the reads via the NCBI website. Sadly it is still
saying "Our database is temporarily unavailable. Please come
back later."

Peter




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