[Biopython] subclassing SeqRecord
Björn Johansson
bjorn_johansson at bio.uminho.pt
Tue Apr 26 15:16:22 UTC 2011
Hi, I tried to subclass the SeqRecord object to make it take a new parameter
which is a int describing the annealing position for a primer. I tried the
code below, but it does not produce the result I want and it is also ugly,
mostly where indicated. This question may be more about Python the
BioPython, but I run into the wall here.
The comments in the code point out where it goes wrong.
I have RTFM but in the Python docs, but I still did not figure this one out.
I suspect that I am complicating things.
If someone knows how to do this, I would be grateful!
cheers,
bjorn
from Bio.Alphabet.IUPAC import ambiguous_dna
from Bio.SeqRecord import SeqRecord
from Bio.Seq import Seq
#Subclassing of of the SeqRecord class
class Primer(SeqRecord):
def __init__(self, primer, annealing_position):
assert type(primer) == SeqRecord
primer.seq.alphabet = ambiguous_dna
SeqRecord.__init__(self,primer,primer.id,primer.name,primer.description)
# Is this how to do it ???
#I want to pass the SeqRecord object given as parameter to my new
primer object
self.annealing_position = annealing_position
a = SeqRecord(Seq("aaa"),"id","name")
print a.seq
# prints aaa
print type(a.seq)
# prints <class 'Bio.Seq.Seq'>
b= Primer(a,33)
print b.annealing_position
# prints 33
print b.seq
#prints
#ID: id
#Name: name
#Description: <unknown description>
#Number of features: 0
#Seq('aaa', IUPACAmbiguousDNA())
print type(b.seq)
#<class 'Bio.SeqRecord.SeqRecord'>
# here I was expection Bio.Seq.Seq
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