[Biopython] example request for using stdin and stdout with 'needle' in EMBOSS

[charlie]

Hi all,

Wonder if anyone can provide an example for using needle but take stdin as
input and stdout as output within biopython.
I did like this, but it doesn't work.

cline = NeedleCoomandline( gapopen=10, gapextend=.5, outfile='stdout',
asequence='stdin', bsequence='stdout')
child = subprocess.Popen( cline, shell=True, stdout=PIPE, stdin = PIPE,
stderr = PIPE )
SeqIO.write( a, child.stdin, 'fasta')
SeqIO.write( b, child.stdin, 'fasta')
child.stdin.close()
print child.returncode

returncode is None

THanks

Li