[BioPython] BLAST subprocess problem with a GUI

[Peter]

On Wed, Feb 25, 2009 at 7:48 AM, Stefanie Lück <lueck at ipk-gatersleben.de> wrote:
> I tried this but after compilation/installation Primer3 gives no output,
> only a return code of -1073741515 (but no errors or messages)...

The error number -1073741515 can be regarded as the hex representation
0xc0000135, which could be "The application failed to initialize
properly" (try searching both in Google).  Without more information
this would be hard to resolve as this seems to be a rather generic
error code.

Is this problem showing up on your own machine or someone elses?  If
it works on some computers and not others, this would suggest its
could be a problem with the primer3 installation rather than your
code.

Does it work if you run the code directly in Python rather than via py2exe?

I would suggest you add a message box or print statement to show the
actual command line string to the user just before trying to run the
program.  Make a note of this, then also try running this command by
hand.  It could be something missing in your EMBOSS setup (e.g. it
can't find certain data files).

Peter