[Biopython] fastq-solexa index

xyz mitlox at op.pl
Wed Dec 30 02:42:07 UTC 2009


Peter wrote:
> In Bio.SeqIO we give each file format a name, in this case "fastq-solexa"
> means the old Solexa FASTQ files (also used by Illumina up to and
> including pipeline 1.2) which use Solexa scores with an ASCII offset
> of 64 (not PHRED scores). The table on the SeqIO wiki page tries to
> summarise this. See also: http://en.wikipedia.org/wiki/FASTQ_format
>
> The "index" column on that table on the SeqIO wiki page indicates if
> each file format can be used with the Bio.SeqIO.index(...) function
> included in Biopython 1.52 onwards. See:
> http://news.open-bio.org/news/2009/09/biopython-seqio-index/
>
> There are also examples in the main Tutorial,
> http://biopython.org/DIST/docs/tutorial/Tutorial.html
> http://biopython.org/DIST/docs/tutorial/Tutorial.pdf
>
> And in the Bio.SeqIO module's built in help, online here:
> http://biopython.org/DIST/docs/api/Bio.SeqIO-module.html
>
> >From within Python:
>
>   
>>>> from Bio import SeqIO
>>>> help(SeqIO)
>>>>         
> ...
>   
>>>> help(SeqIO.index)
>>>>         
> ...
>
> Peter
>
>   
Thank you.



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