[Biopython-dev] [Biopython - Feature #3341] (New) Improve SffIO to parse 3 extra lines present in some SFF files ("Run Name:, Analysis Name:, Full Path:)"
redmine at redmine.open-bio.org
redmine at redmine.open-bio.org
Thu Apr 12 14:52:35 EDT 2012
Issue #3341 has been reported by Martin Mokrejš.
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Feature #3341: Improve SffIO to parse 3 extra lines present in some SFF files ("Run Name:, Analysis Name:, Full Path:)"
https://redmine.open-bio.org/issues/3341
Author: Martin Mokrejš
Status: New
Priority: Normal
Assignee:
Category:
Target version:
URL:
Some file have extra 3 lines per each record in the SFF file. One such file is already in biopython test data:
biopython/Tests/Roche/E3MFGYR02_random_10_reads.sff
biopython/Tests/Roche/paired.sff
The three lines "Run Name:, Analysis Name:, Full Path:" are not parsed into the object and later on, are not written out. Hence, sff round trip read in -> write out breaks (biopython-1.58). These three lines somehow do not appear in every SFF file, and so far I haven't seen these in files extracted from SRA. Seems these only appear in original Roche SFF files.
>E3MFGYR02JWQ7T
Run Prefix: R_2008_01_09_16_16_00_
Region #: 2
XY Location: 3946_2103
Run Name: R_2008_02_08_17_05_24_build11_mlabrecque_100707593662420SV11007SID4607RunID24947331
Analysis Name: /data/2008_02_08/R_2008_02_08_17_05_24_build11_mlabrecque_100707593662420SV11007SID4607RunID24947331/D_2008_02_08_23_45_24_d41_AnalysisPipe
Full Path: /data/R_2008_02_08_17_05_24_build11_mlabrecque_100707593662420SV11007SID4607RunID24947331/D_2008_02_08_23_45_24_d41_AnalysisPipe
Read Header Len: 32
Name Length: 14
# of Bases: 265
Clip Qual Left: 5
Clip Qual Right: 264
Clip Adap Left: 0
Clip Adap Right: 0
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