[Biopython-dev] [Bug 2848] SeqIO fastq routines reject valid quality socres

bugzilla-daemon at portal.open-bio.org bugzilla-daemon at portal.open-bio.org
Wed Jun 3 21:39:07 UTC 2009


http://bugzilla.open-bio.org/show_bug.cgi?id=2848





------- Comment #1 from sbassi at gmail.com  2009-06-03 17:39 EST -------
Seems that is intented, look at the module docs:

The PHRED software reads DNA sequencing trace files, calls bases, and
assigns a quality value between 0 and 90 to each called base using a logged
transformation of the error probability, Q = -10 log10( Pe ), for example::

    Pe = 0.0,         Q =  0
    Pe = 0.1,         Q = 10
    Pe = 0.01,        Q = 20
    ...
    Pe = 0.00000001,  Q = 80
    Pe = 0.000000001, Q = 90


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