[Biopython-dev] [Bug 2848] SeqIO fastq routines reject valid quality socres
bugzilla-daemon at portal.open-bio.org
bugzilla-daemon at portal.open-bio.org
Wed Jun 3 21:39:07 UTC 2009
http://bugzilla.open-bio.org/show_bug.cgi?id=2848
------- Comment #1 from sbassi at gmail.com 2009-06-03 17:39 EST -------
Seems that is intented, look at the module docs:
The PHRED software reads DNA sequencing trace files, calls bases, and
assigns a quality value between 0 and 90 to each called base using a logged
transformation of the error probability, Q = -10 log10( Pe ), for example::
Pe = 0.0, Q = 0
Pe = 0.1, Q = 10
Pe = 0.01, Q = 20
...
Pe = 0.00000001, Q = 80
Pe = 0.000000001, Q = 90
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