[Bioperl-l] Bioperl clustalw-align not recognized

[Anil Kumar]

Hi Forum members...

When i tried to align two sequence using clustalW perl module (in windows),
it shows error message as follows....So kindly suggest me some solution.

Ani Lee,
Seoul



"align is not recognized as internal or external command

etc etc..

MSG: ClustalW call ( align  -infile=seq.txt -output=gcg   -matrix=BLOSUM
-ktuple
=3 -outfile=C:\DOCUME~1\chirag\LOCALS~1\Temp\rIC8w2_4Ag\5JCfhbka_j 2>&1)
crashed
: 256
STACK Bio::Tools::Run::Alignment::Clustalw::_run
C:/Perl/lib/Bio/Tools/Run/Align
ment/Clustalw.pm:767
STACK Bio::Tools::Run::Alignment::Clustalw::align
C:/Perl/lib/Bio/Tools/Run/Alig
nment/Clustalw.pm:515
STACK toplevel C:\PROGRA~1\LUCKAS~1\ENGINS~1\data\CLUSTA~1.PL:33