[Bioperl-l] PRIMER_THERMODYNAMIC_PARAMETERS_PATH

Veronica A. armendarez77 at hotmail.com
Wed Apr 17 12:32:53 EDT 2013


Hello,
I've just updated to bioperl-run (1.006900) and perl (v5.14.2) installed on a Linux machine running Ubuntu 12.04.
I have perl scripts that use Bio::Tools::Run::Primer3 and before the updates, ran as expected.  However, they are now failing.
While testing primer3_core on the command line, I realized the problem was the PRIMER_THERMODYNAMIC_PARAMETERS_PATH setting.  I added this to my primer3_core input file and primers were designed.  I.T. then moved the primer3_config directory to /opt/primer3_config as instructed and this helped running primer3_core on the command line as I no longer needed to include PRIMER_THERMODYNAMIC_PARAMETERS_PATH to the input file.  However, the perl scripts still do not work.
Is there a BioPerl config file that requires the PRIMER_THERMODYNAMIC_PARAMETERS_PATH setting?  Or did something not install correctly?
Thank you,
Veronica 		 	   		  



More information about the Bioperl-l mailing list