[Bioperl-l] Working with GenBank file

Shyam Saladi saladi1 at illinois.edu
Tue May 29 14:11:23 EDT 2012


Hi,

I want to extract certain genes from a genomic genbank file. I put together
the following, but get the error:

------------- EXCEPTION: Bio::Root::Exception -------------
MSG: asking for tag value that does not exist translation
STACK: Error::throw
STACK: Bio::Root::Root::throw
/usr/local/share/perl/5.10.1/Bio/Root/Root.pm:472
STACK: Bio::SeqFeature::Generic::get_tag_values
/usr/local/share/perl/5.10.1/Bio/SeqFeature/Generic.pm:522
STACK: readfromgbk.pl:91
-----------------------------------------------------------

@cds_features = grep { $_->primary_tag eq 'CDS' } Bio::SeqIO->new(-file =>
$inFile)->next_seq->get_SeqFeatures;
my %gene_sequences = map {$_->get_tag_values('gene'),
$_->get_tag_values('translation')} @cds_features;

I think the error has to do with ->get_SeqFeatures, but don't understand
what exactly.

Could someone please advise?

Thanks very much,
Shyam



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