[Bioperl-l] Fishing redundant sequences in FASTA files [Right formatting]
Juan Jovel
jovel_juan at hotmail.com
Tue Feb 15 14:34:58 EST 2011
Good Morning guys,
> sorry for the naive question: What's the simplest way to fish redundant sequences (complete or partial) between two (or more) fasta files.
> I was thinking just to do it with SeqIO, opening two files, and compare each sequence of file_1 to each record of file_2, like:
# Read each record of file 1 and compare to each read of
file 2
while(my $dna1 = $seqin1->next_seq){
my $seq1 =
$dna1->seq;
my $id1 =
$dna1->id;
# Iterate
inside de second fasta file
while(my $dna2
= $seqin2->next_seq){
my $seq2 = $dna2->seq;
my
$id2 = $dna2->id;
if(($seq1 =~ /$seq2/)||($seq2 =~ /$seq1/)){
print "Match found \n";
print OUT "Records $id1 and $id2 are redundants";
I am afraid it is going to be slow for large files. AND, more importantly, how do I reset the object containing the second file to the first line, as done in Perl with (SEEK(IN, 0,0)) for example. Does SeqIO allows that (sorry, I am not a frequent user of SeqIO). If there is another more-elaborated module to fish such redundant sequences, I will appreciate to know.
Thanks,
JUAN
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