[Bioperl-l] SeqIO: paired end reads
Lee Katz
lskatz at gmail.com
Wed Aug 3 21:01:24 UTC 2011
Hi all! I was wondering how to construct paired end reads from scratch. I
know the locations of certain sequences across the genome with a high degree
of confidence and so I want to give them to my assembler as paired end
reads, along with my other sequence runs (454 and Illumina runs). I plan to
use Newbler.
My only problem is that I do not know the correct format in order to specify
distance and sequences for a paired end reads run, and so I hope that there
is a SeqIO solution. At the least, I hope that one bioperl member can point
me to where the definition of the paired end reads file format is...?
Thank you!
--Lee
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