[Bioperl-l] SeqIO::abi use

[Joel Martin]

I'd use phred instead of kbcaller, and with the -d for .poly files 
option, then parse the .poly files,
sounds like it might be the information you're after.

Joel

Tom Keller wrote:
> Greetings,
> Can the Bio::SeqIO::abi module be used to access the raw fluorescence data? Are there any examples available? I'm wondering how to calculate ratios of mixed bases prior to the smoothing and scaling done by the base caller.
>
> thank you
> Tom
> kellert at ohsu.edu<mailto:kellert at ohsu.edu>
> 503-494-2442
>
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