[Bioperl-l] question about Bio::Tools::Run::StandAloneBlast

[Dimitar Kenanov]

Hi guys,
another question, this time about "Bio::Tools::Run::StandAloneBlast" . 
Now i want to run 'tblastn', so i follow the guide on CAPN but it doesnt 
matter what i do the module complains:

--------------------- WARNING ---------------------
MSG: cannot find path to blastall

This is frustrating :) Here is my code:

##########
my $report_tbn="report_TBN_out.txt";
my $dbase="/path/to/Preformatted-DB/human_genomic";
my @params_tbn = (-program => 'tblastn',
                                      -database => "$dbase",
                                      -outfile => "$tmpout");

my $factory_tbn = Bio::Tools::Run::StandAloneBlast->new(@params_tbn);
$factory_tbn->e("0.01");
$factory_tbn->program_dir("/opt/blast/bin");_#here i tried to help it :)_
$factory_tbn->executable('tblastn');

my $seq_stream = Bio::SeqIO->new(-file=>"$report_ss", -format => 'Fasta');
my $seq2;
while($seq2=$seq_stream->next_seq()){
     my $blast_report = $factory_tbn->blastall($seq);
}
##############

May be the problem is that i am using BLAST+ package where theres no 
'blastall' at all and 'tblastn' and all programs are standalone.
If someone can guide me where(which module) i have to tweak a bit in 
order to drive that programs i would be grateful :)
Thank you for any help or directions

Cheers
Dimitar

-- 
Dimitar Kenanov
Postdoctoral research fellow
Protein Sequence Analysis Group
Bioinformatics Institute
A*STAR, Singapore
email: dimitark at bii.a-star.edu.sg
tel: +65 6478 8514