[Bioperl-l] looks like a Bio::SeqIO error
Chris Fields
cjfields at illinois.edu
Fri May 15 18:42:01 UTC 2009
As mentioned in the thread by Kevin, using LargeSeq may not be the
best route as it generates a tmpdir/tmpfile for each sequence, so you
are probably reaching a hard limit for the number of temp files for
your system or for File::Temp. The oddity is one or the other (or
both) are not removed, so I think this is a legit bug.
Regardless, there's a good reason this hasn't been encountered
before. If you have ~30,000 sequences that you extract subseqs from
you should seriously consider using a flat file db such as
Bio::DB::Fasta or similar. It is capable of handling very large
sequences or files and can extract subseqs easily.
chris
On May 15, 2009, at 12:12 PM, fungazid wrote:
>
> After installing XML-SAX module, the my installation of bioperl 1.6
> was
> successful (I didn't need to force install).But the error I reported
> previously (Too many links at /usr/local/share/perl/5.8.8/Bio/Root/
> IO.pm
> line 740) still exists.
>
> --
> View this message in context: http://www.nabble.com/looks-like-a-Bio%3A%3ASeqIO-error-tp23559474p23563950.html
> Sent from the Perl - Bioperl-L mailing list archive at Nabble.com.
>
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