[Bioperl-l] Creating a fastq format file?

[Hotz, Hans-Rudolf]

> Howdy
> 
> This might be a good place to ask the question: having looked at the
> fastq.pm page, is the fastq format defined (only) by a "@'" followed
> by a sequence line and a "+" header followed by a quality line and the
> two headers have to agree? Now that Illumina is using phred scaling,
> are 'Sanger' and 'Illumina' versions the same?

No, see:
http://maq.sourceforge.net/fastq.shtml

Regards, Hans


> 
> Thanks
> 
> Mike