[Bioperl-l] coloring of HSPs in blast panel
Smithies, Russell
Russell.Smithies at agresearch.co.nz
Tue Oct 2 21:05:25 EDT 2007
Hi all,
I'm using a modified version of Lincoln's tutorial
(http://www.bioperl.org/wiki/HOWTO:Graphics#Parsing_Real_BLAST_Output)
and I'm colouring the HSPs by setting the -bgcolor by score with a sub
to give a similar image to that from NCBI but for some reason, my
colours are coming out wrong (see attached example)
They seem to be off by one but I can't see why.
Any ideas?
I can't be certain but I think it's only started doing this since our
BLAST upgrade to 2.2.17 a few weeks ago.
Here's the colouring code:
------------------------------------------------------------------------
-------
my $track = $panel->add_track(
-glyph => 'segments',
-label => 1,
-connector => 'dashed',
-bgcolor => sub {
my $feature = shift;
my $score = $feature->score;
return 'red' if $score >= 200;
return 'fuchsia' if $score >= 80;
return 'lime' if $score >= 50;
return 'blue' if $score >= 40;
return 'black';
},
-font2color => 'gray',
-sort_order => 'high_score',
-description => sub {
my $feature = shift;
return unless
$feature->has_tag('description');
my ($description) =
$feature->each_tag_value('description');
my $score = $feature->score;
"$description, score=$score";
},
);
------------------------------------------------------------------------
---------
Thanx,
Russell Smithies
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