[Bioperl-l] not getting all exons back when using Bio::DB::GFF
Niels Klitgord
niels_klitgord at dfci.harvard.edu
Thu Mar 9 10:23:44 EST 2006
Hello,
Perhaps this is because I'm not using Bio::DB::GFF correctly. Perhaps
I missed a previous post on this, if so I apologize. But on occasion
when I try to get all the exons of an orf from a segment I wind up
missing 1. I am using wormbase WS150 release GFF, and am using
GFF.pm,v 1.102 perl modual (maybe I need to upgrade?).
This is the code I was using:
#!/usr/local/bin/perl -w
use strict;
use Bio::DB::GFF;
my $GFFdb = new Bio::DB::GFF(-adaptor=>'dbi::mysqlopt',
-dsn=>'dbi:mysql:gff150;host=dome',
-user=>'niels')or die("can't open gffDB");
my $gene = 'C08C3.3';
my @seg = $GFFdb->segment(-name=>$gene, -class=>'CDS');
print "$gene CDS: ", $seg[0]->abs_start, "\t", $seg[0]->abs_stop, "\n";
my @all_exons = $seg[0]->features( 'exon:curated' );
foreach my $k (sort { $a->start <=> $b->start} @all_exons) {
print "feature: ", $k->class, "\t", $k->type, "\t", $k->name, "\t",
$k->abs_start, "\t", $k->abs_stop, "\n"
}
and get:
C08C3.3 CDS: 7783311 7777192
feature: CDS exon:curated C08C3.3 7782898 7782816
feature: CDS exon:curated C08C3.3 7782130 7782027
feature: CDS exon:curated C08C3.3 7778462 7778314
feature: CDS exon:curated C08C3.3 7777314 7777192
However in the raw gff file we see (and also in the mysql ):
CHROMOSOME_III curated exon 7777192 7777314 . - . CDS
"C08C3.3" <found>
CHROMOSOME_III curated exon 7778314 7778462 . - . CDS
"C08C3.3" <found>
CHROMOSOME_III curated exon 7782027 7782130 . - . CDS
"C08C3.3" <found>
CHROMOSOME_III curated exon 7782816 7782898 . - . CDS
"C08C3.3" <found>
CHROMOSOME_III curated exon 7783168 7783311 . - . CDS
"C08C3.3" <not found!!>
Am I just using this wrong, or should the last entry be returned as well?
Much thanks in advance,
Niels
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