[Bioperl-l] StandAloneBlast in Windows

[Bornman, Daniel M]

Dear BioPerl,


Does anyone know how to use "Bio::Tools::Run::StandAloneBlast" in
Windows?

I am able to query my own stand alone blast database from the command
line directly without perl so I know its installed correctly but I am
unable to get my BioPerl script to work.  Here is a very simple program
example and the error message I get.  
My Path (Environmental Variable) is set to 'C:\BLASTDB\bin'



## START ##
BEGIN{
	$ENV{BLASTDIR} = 'C:\\BLASTDB\\data';
}

use strict;
use Bio::Seq;
use Bio::Tools::Run::StandAloneBlast;

my @params = (program  => 'blastn', database => 'ecoli.nt');
my $blast_obj = Bio::Tools::Run::StandAloneBlast->new(@params);
my $seq_obj = Bio::Seq->new(-id  =>"testquery", -seq
=>"TTTAAATATATTTTGAAGTATAGATTATATGTT");
my $report_obj = $blast_obj->blastall($seq_obj);
my $result_obj = $report_obj->next_result;
print $result_obj->num_hits;
## END ##


## ERROR MESSAGE START ##

[NULL_Caption] WARNING: testquery: Unable to open ecoli.nt.nin

------------- EXCEPTION  -------------
MSG: blastall call crashed: 256 C:\BLASTDB\bin\blastall.exe -p  blastn
-d  "\ecoli.nt"  -i  C:\DOCUME~1\Home\LOCALS~1\Temp\QZwYdo9eKx  -o
C:\DOCUME~1\Home\LOCALS~1\Temp\ADP2OcZw8z 

STACK Bio::Tools::Run::StandAloneBlast::_runblast
C:/Perl/site/lib/Bio/Tools/Run/StandAloneBlast.pm:732
STACK Bio::Tools::Run::StandAloneBlast::_generic_local_blast
C:/Perl/site/lib/Bio/Tools/Run/StandAloneBlast.pm:680
STACK Bio::Tools::Run::StandAloneBlast::blastall
C:/Perl/site/lib/Bio/Tools/Run/StandAloneBlast.pm:536
STACK toplevel localblast.pl:12

--------------------------------------

## ERROR MESSAGE END ##


Any help would be greatly appreciated.

Thanks,
Dan



Daniel Bornman
Researcher
Battelle Memorial Institute
505 King Ave
Columbus, OH 43201
614.424.3229