[Bioperl-l] Bio:Tools::Primer3, Bio::Seq::PrimedSeq, and Bio: :SeqFeature::Primer

anthony.underwood at hpa.org.uk anthony.underwood at hpa.org.uk
Fri Apr 4 17:13:41 EST 2003


Dear Rob,

Thankyou very much for putting together the primer3 modules for bioperl.
They really are extremely useful. However I've run into a problem with the
Run/Primer3.pm module

At line 341
 print $temphandle join "\n", @{$self->{'primer3_input'}}, "=\n";

This doesn't produce any data in the temporary file and none of the test
scripts work due to primer3 failing  However if I replace it with the text
below then all tests work.


 open (INPUT, ">$tempfile");
 print INPUT join "\n", @{$self->{'primer3_input'}}, "=\n";
 close INPUT;


Is this is a setup problem with my machine?

Thanks for our help,


Anthony
Dr Anthony Underwood
Bioinformatics Unit
Central Public Health Laboratory
61 Colindale Avenue
London
NW9 5HT
t:    0208 2004400 ext. 3618
f:    0208 3583138
e: aunderwood at phls.org.uk
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