[Bioperl-l] writing genbank files
gert thijs
gert.thijs@esat.kuleuven.ac.be
Wed, 18 Sep 2002 10:48:17 +0200
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Hilmar,
I just installed the modules from the main trunk. I tried to test it but now I
was unable to parse input sequences in genbank format.
Now I have a problem uploading a genbank flat file. There seems to be a
problem while parsing the species name. I guess not having an upper case
starting letter stops the genbank parser. In attachment you can find a file on
which the parser throws the expection.
------------- EXCEPTION: Bio::Root::Exception -------------
MSG: Invalid name 'eurosids II' (Wrong case?)
STACK: Error::throw
STACK: Bio::Root::Root::throw
/users/sista/thijs/perl/lib/site_perl/5.6.0/Bio/Root/Root.pm:318
STACK: Bio::Species::validate_name
/users/sista/thijs/perl/lib/site_perl/5.6.0/Bio/Species.pm:283
STACK: Bio::Species::classification
/users/sista/thijs/perl/lib/site_perl/5.6.0/Bio/Species.pm:121
STACK: Bio::SeqIO::genbank::_read_GenBank_Species
/users/sista/thijs/perl/lib/site_perl/5.6.0/Bio/SeqIO/genbank.pm:884
STACK: Bio::SeqIO::genbank::next_seq
/users/sista/thijs/perl/lib/site_perl/5.6.0/Bio/SeqIO/genbank.pm:229
STACK: AnnotatedSequence::new /users/sista/thijs/perl/lib//AnnotatedSequence.pm:66
STACK: GeneIndex.pl:168
-----------------------------------------------------------
Gert
Hilmar Lapp wrote:
> It should be written as join(complement(...),complement(...),...). This
> is main trunk only though. Do you have an example where this is not true?
>
> -hilmar
>
> On Tuesday, September 17, 2002, at 02:06 AM, gert thijs wrote:
>
>> Hello,
>>
>> I have a question about the current status of the genbank file
>> parser/writer. I noticed that a CDS with a location of the type
>> complement(join()) is written as a join() without the complement.
>> I saw that this problem has been a major thread on the list a few
>> weeks ago, but I could not find if the problem has been solved by now
>> or if it was solved how it should be solved.
>>
>> Gert
>>
>>
>>
>> -- + Gert Thijs
>> + K.U.Leuven
>> + ESAT-SCD
>> + Kasteelpark Arenberg 10
>> + B-3001 Leuven-Heverlee
>> + Belgium
>> +
>> + Tel : +32 16 32 85 88
>> + Fax : +32 16 32 19 70
>> + email: gert.thijs@esat.kuleuven.ac.be
>> +
>> + http://www.esat.kuleuven.ac.be/~thijs
>> + http://www.esat.kuleuven.ac.be/~dna/BioI/
>> +
>>
>> _______________________________________________
>> Bioperl-l mailing list
>> Bioperl-l@bioperl.org
>> http://bioperl.org/mailman/listinfo/bioperl-l
>>
> --
> -------------------------------------------------------------
> Hilmar Lapp email: lapp at gnf.org
> GNF, San Diego, Ca. 92121 phone: +1-858-812-1757
> -------------------------------------------------------------
>
> _______________________________________________
> Bioperl-l mailing list
> Bioperl-l@bioperl.org
> http://bioperl.org/mailman/listinfo/bioperl-l
>
--
+ Gert Thijs
+ K.U.Leuven
+ ESAT-SCD
+ Kasteelpark Arenberg 10
+ B-3001 Leuven-Heverlee
+ Belgium
+
+ Tel : +32 16 32 85 88
+ Fax : +32 16 32 19 70
+ email: gert.thijs@esat.kuleuven.ac.be
+
+ http://www.esat.kuleuven.ac.be/~thijs
+ http://www.esat.kuleuven.ac.be/~dna/BioI/
+
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LOCUS AB000094 15532 bp DNA linear PLN 13-JAN-1998
DEFINITION Arabidopsis thaliana gene for inorganic phosphate transporter,
protein phosphatase 1 catalytic subunit, complete cds.
ACCESSION AB000094
VERSION AB000094.1 GI:2780346
KEYWORDS protein phosphatase 1 catalytic subunit inorganic phosphate
SOURCE Arabidopsis thaliana (strain:Columbia) DNA.
ORGANISM Arabidopsis thaliana
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; eudicotyledons; core eudicots;
Rosidae; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
REFERENCE 1 (bases 0 to 0)
AUTHORS Mitsukawa,N., Okumura,S. and Shibata,D.
TITLE High-affinity phosphate transporter genes of Arabidopsis thaliana
JOURNAL Soil Sci. Plant Nutr. 43, 971-974 (1997)
REFERENCE 2 (bases 0 to 0)
AUTHORS Mitsukawa,N., Okumura,S. and Shibata,D.
TITLE Isolation of a gene that encode a protein phosphatase 1 catalytic
subun it in Arabidopsis thaliana
JOURNAL Unpublished (1996)
REFERENCE 3 (bases 1 to 15532)
AUTHORS Mitsukawa,N.
TITLE Direct Submission
JOURNAL Submitted (24-DEC-1996) to the DDBJ/EMBL/GenBank databases.
Norihiro Mitsukawa, Mitsui Plant Biotech.Res.Inst., Research
Division; TCI-D21, Sengen 2-1-6, Tsukuba, Ibaraki 305, JAPAN
(E-mail:tsu01129@koryu.statci.go.jp, Tel:0298-58-6252,
Fax:0298-58-6234)
COMMENT Sequence updated (08-Jan-1998).
FEATURES Location/Qualifiers
source 1..15532
/chromosome=5
/strain="Columbia"
/organism="Arabidopsis thaliana"
/db_xref="taxon:3702"
gene join(2560..2960,3086..4250)
/gene="PHT3"
CDS join(2560..2960,3086..4250)
/product="inorganic phosphate transporter"
/gene="PHT3"
/protein_id="BAA24281.1"
/codon_start=1
/translation="MADQQLGVLKALDVAKTQLYHFTAIVIAGMGFFTDAYDLFCVSL
VTKLLGRLYYFNPTSAKPGSLPPHVAAAVNGVALCGTLAGQLFFGWLGDKLGRKKVYG
ITLIMMILCSVASGLSLGNSAKGVMTTLCFFRFWLGFGIGGDYPLSATIMSEYANKKT
RGAFIAAVFAMQGVGILAGGFVALAVSSIFDKKFPSPTYEQDRFLSTPPQADYIWRII
VMFGALPAALTYYWRMKMPETARYTALVAKNIKQATADMSKVLQTDLELEERVEDDVK
DPKKNYGLFSKEFLRRHGLHLLGTTSTWFLLDIAFYSQNLFQKDIFSAIGWIPKAATM
NAIHEVFKIARAQTLIALCSTVPGYWFTVAFIDIIGRFAIQLMGFFMMTVFMFAIAFP
YNHWILPDNRIGFVVMYSLTFFFANFGPNATTFIVPAEIFPARLRSTCHGISAATGKA
GAIVGAFGFLYAAQPQDKTKTDAGYPPGIGVKNSLIMLGVINFVGMLFTFLVPEPKGK
SLEELSGEAEVDK"
/db_xref="GI:2780347"
gene join(9110..9510,9661..10834)
/gene="PHT2"
CDS join(9110..9510,9661..10834)
/product="inorganic phosphate transporter"
/gene="PHT2"
/protein_id="BAA24282.1"
/codon_start=1
/translation="MAEQQLGVLKALDVAKTQLYHFTAIVIAGMGFFTDAYDLFCVSL
VTKLLGRIYYFNPESAKPGSLPPHVAAAVNGVALCGTLSGQLFFGWLGDKLGRKKVYG
LTLIMMILCSVASGLSFGNEAKGVMTTLCFFRFWLGFGIGGDYPLSATIMSEYANKKT
RGAFIAAVFAMQGVGILAGGFVALAVSSIFDKKFPAPTYAVNRALSTPPQVDYIWRII
VMFGALPAALTYYWRMKMPETARYTALVAKNIKQATADMSKVLQTDIELEERVEDDVK
DPRQNYGLFSKEFLRRHGLHLLGTTSTWFLLDIAFYSQNLFQKDIFSAIGWIPKAATM
NATHEVFRIARAQTLIALCSTVPGYWFTVAFIDTIGRFKIQLNGFFMMTVFMFAIAFP
YNHWIKPENRIGFVVMYSLTFFFANFGPNATTFIVPAEIFPARLRSTCHGISAAAGKA
GAIIGAFGFLYAAQNQDKAKVDAGYPPGIGVKNSLIVLGVLNFIGMLFTFLVPEPKGK
SLEELSGEAEVSHDEK"
/db_xref="GI:2780348"
CDS join(13719..13967,14046..14605,14688..14862)
/product="protein phosphatase 1 catalytic subunit"
/protein_id="BAA24283.1"
/codon_start=1
/translation="MDPGTLNSVINRLLEAREKPGKIVQLSETEIKQLCFVSRDIFLR
QPNLLELEAPVKICGDIHGQYPDLLRLFEHGGYPPNSNYLFLGDYVDRGKQSLETICL
LLAYKIKFPENFFLLRGNHESASINRIYGFYDECKRRFSVKIWRIFTDCFNCLPVAAL
IDERIFCMHGGLSPELLSLRQIRDIRRPTDIPDRGLLCDLLWSDPDKDVRGWGPNDRG
VSYTFGSDIVSGFLKRLDLDLICRAHQVVEDGFEFFANKQLVTIFSAPNYCGEFDNAG
AMMSVSEDLTCSFQILKSNDKKSKFSFGSRGGAKTSFPYPKVKVCINHITF"
/db_xref="GI:2780349"
BASE COUNT 5197 a 2674 c 2593 g 5068 t
ORIGIN
1 cttttaaaaa atttacaaag atttttaaaa gcattttttt ggctgggaaa aaaattttac
61 gagaaaacat ttttggcggg aaattttttt tttggcggga aaaaaagttt ggcggaaatt
121 ttttgtttgg cgggaaaaat aaattttggt acatgttatt attaaatgag ggtaatttgg
181 tcattctgtt caatagaagg gatattttta aaaataaaca atataaaatg gtattgttac
241 aaaagggtag taaaaaaagg gtagttttgc aaatctccct cgagaatttg caatttctct
301 ctcatagcat aacatgtctt ttttttagta aatttcaaga tgacaaaaag aaaaaaaaaa
361 cataaagaaa caaattgaaa gcaacatcta tttttctgac ttgtaaaaat gtggtgttac
421 tagcaaatat caaatttttg tatctataaa ctagatttta acccgcggta tactaggaga
481 acgatttatt ttttaaagtt aatatatata caagtttaca aattgaatat atttataaaa
541 aaaataaatt ttttagttta caattattat taggtaacat cccgccaaat ctgttccatc
601 aaaaagctta ttaattttat taatgttaac cttagttatg atatgattct aaatcattgt
661 ctagatattt tagccaatgt taggacgttg aacccacata gttttcgtca attgaataat
721 atatgttcgt tttataaatt tcgaatcaca atttatgcag aaaatgtttt gaaatttttc
781 ttaactttac atattataca ataaaaaatc aaacaaagat gataggaaat tcatagtttt
841 aaatcttaat caaatcttct aaatatcaat attgttaaat atagaagtaa tgagtataag
901 agttgggttt tatttgaacc aacccaacta agatttaatg aacatcataa actaatgggt
961 ctggtatcaa atgtaatcag atgcatttta acccacaatc aaaaggtgac tgcaaaaata
1021 ctatataaga ttcatagaag tagatcgtct atataggcct gaaaaatatc agttttaaat
1081 taaattattt agagatctta ttttgttggg cttaaatact ggagaataag actactttag
1141 taatcaaaat atttgggcca tgtaactaat gcaaaccaaa atcattattg acaaaagtca
1201 ttgcatcaaa tgattttctt accaaaatgt ctttaaaata tatatatata tatatatata
1261 tatatgtacc ttcgtacggt acggttgctt ttatataaaa aggtgttgta cggttgcatt
1321 gacttttgat cgtaaatact atatagtccg gagactcaaa tgcaaaccac tttctctatt
1381 tttttggacg gaagttatgt taacctaaat tatctaaaat attacacaaa taatttttaa
1441 acagtttaaa atggcatata attatgttaa tagaaatttg acttttagta cggagataca
1501 ttagctgaga agtttgacct caccttattt agtgggaacc ttgttacttt tgaattagtt
1561 tgaacaatat atctctaatt catataaagt attttgaaat ataaagcttt ataaatataa
1621 catatttttt ggtgggtaat gcttcttttt ttctgtggct tataactacg tggagatctt
1681 atcttctgtc cgcttatatt agtatttcac catagtgatt ttaataaaaa tcagattaaa
1741 caatatataa cagctatttt agtttacagg tttaaacttt tattttagtt ctttgatttt
1801 ttttttcttt cagaaaatgg tggacatctt ctaaagtttg ttttattaat ctaggtagaa
1861 actttcaaac aaaagacgaa aacagaaaag ttgactttag tacggaaata catgatatga
1921 gataactgat aagataactt gttagatggt ttccactctc ataaacaata taaaaaaatt
1981 attaacgata gtgaactcga tttttagtaa ctctatgaaa atcttcttgt gagatcataa
2041 cattttagaa gactaggctt ttcgtgtaat atgatctgta gaccattcca atgttatcat
2101 atgcagaaca gcaacacaat gaaataaatt aagagatgga aaataaatta agatcaaata
2161 aaatgtggat atacccttaa gattccggag acaatacttc tccagttctc ctctcgtcta
2221 taaatttctg ccgtctcagc caacataatc acaaccacca cctctctcaa taattctctc
2281 tcttgtcctt cacaacctgt tactacttag gtgaatatca tctacatgtt tattttgtct
2341 taatccaaat tcacatttaa ttttgttttc actcttttta tcatgtatac ttttttaatt
2401 agctagccga gattatatat gttaaaacta cctctatttc taacacaccc aagcaatata
2461 tcatctttcc aatatttacg aaagaagaaa tttctataca aatattaacc tttactttgt
2521 gcaaaatagg agagaagaaa aacaaaaaga ctgactgaga tggccgatca acagctagga
2581 gtgctaaagg cacttgatgt tgcgaagacg caactttacc atttcacggc tattgtcatt
2641 gccggtatgg gcttctttac ggacgcgtac gatctctttt gtgtgtcctt ggtgaccaag
2701 cttcttggcc gcctctacta cttcaatcca acgtcagcaa agcctggctc acttccccct
2761 catgttgcgg ctgcagtcaa cggtgtggcc ctttgtggaa cccttgccgg tcaactcttc
2821 ttcggatggc ttggtgacaa actcggacgg aaaaaggtgt acggtatcac tttgatcatg
2881 atgattctct gctcagttgc ttccggtctt tccttgggca attcggccaa gggtgtcatg
2941 acgactcttt gcttcttcag gtacaattta tttagccaca aacctaatat cacatacgtc
3001 acagatacaa gctcgagaga ttagtcacta tttcgaccta gattatggtt acttaagata
3061 ctgatatcta gacgattata tataggtttt ggctcgggtt tggcattgga ggtgactacc
3121 ctctatctgc caccatcatg tctgaatacg ctaacaagaa gactcgtggg gctttcatcg
3181 cggcagtgtt cgccatgcaa ggtgtaggta tcttggcggg aggttttgtg gcacttgcag
3241 tttcttccat atttgacaaa aagttcccat cgccgacgta tgagcaagac aggtttctat
3301 caacgcctcc tcaagctgat tacatttggc gaatcatcgt catgtttggt gctttacccg
3361 cagctttgac ttactattgg cgtatgaaga tgcctgaaac agcccgttac accgctttag
3421 ttgccaagaa catcaaacaa gccacagcag acatgtccaa ggtcttacaa acagatctcg
3481 agcttgagga aagggtggag gatgacgtca aggaccccaa aaaaaactat ggcttgttct
3541 ccaaggaatt ccttagacgc catgggcttc atctccttgg gactacctcc acttggtttt
3601 tgcttgacat cgccttctac agccaaaact tgttccaaaa ggatattttc tcggccattg
3661 gatggatccc aaaggcagcc actatgaacg ccatccatga ggttttcaag attgctaggg
3721 ctcagactct cattgccctc tgcagtacag tcccaggtta ctggttcaca gtagccttta
3781 ttgatatcat tggaaggttt gcgatccaac taatgggatt tttcatgatg accgttttta
3841 tgtttgctat tgccttccca tacaaccact ggattttacc agataatcgt atcggattcg
3901 tggttatgta ctcactcaca tttttcttcg ccaactttgg acccaatgca actactttca
3961 ttgtcccagc tgaaatcttt ccagcaaggc taaggtctac gtgccatgga atatcagccg
4021 caactggtaa ggctggagcc atcgttggag ccttcgggtt cctatatgct gctcaaccac
4081 aggataagac caagacagac gcaggatacc caccgggcat cggagtcaag aactcattga
4141 tcatgcttgg tgtcattaac tttgttggta tgctcttcac cttcctcgtc cctgagccca
4201 agggcaagtc ccttgaagaa ctctccggcg aggctgaggt tgataaatga ttatgccgtc
4261 atatatgttt gtcattggtt ttgcgatgtg tgaattatat ttgtaatggt gtactacttt
4321 tacgttttac gttctttgcg atgagtgaat tatatttgta acggtgtact actttcgctt
4381 tttgtttaaa tgtgtgtgca agtgcaactt gttaagatgt aaactcatgt tatggtcata
4441 tcctagtaat gctataagtt tggaagcaat aaagacatga acaatcaaac aaaaaatatg
4501 cttagtggaa agtttgaaat gaaagatata agggctagtg gatgtaattc tagggatcaa
4561 tattgataga actgataata caaaaaatca gcttgtactg gtatattagg ttgaaaagat
4621 aaatgagata ttttagaact actgaactat aaagattggt atattgttgt tcatgtaatc
4681 gaaagatttt ttttgtaaga ttgtagatga atttatcatc aaatgaacta caaagactag
4741 ggactcccaa gagtccaaga ccctactctt cacttgtcta aacatttaag tgaagcaatg
4801 tcaaattagg agtatgatgc aacagtctat ttgatatatt tgaccagttg tactagactc
4861 aatttgatgc tgccagtgtg ccgtagaagc cttccaaata gacattatag ctttagtcaa
4921 agcaaatgag gaagccgagt ggtttcagaa cttgaaaata tttcgatggg agaaaccagt
4981 gttggcacta cgtgtaaact aaactctgat agcaattatt tattgttcga gcattgcgct
5041 cttagtttaa tgttaaatct tgtctaatta gaagacaaca caaaaaccaa tagcaactaa
5101 tctcaaatag tgtaatctca cttgattata tcaaatcgga tgataaccta tgacctattt
5161 tactaaaagg tctgtcacaa aagtaagtta tatatattca aaacaaaaaa aatataagat
5221 aaaaaataaa acgaagaaaa actgggaatg tcaaatatga taaatgattt aaacaaatat
5281 gtcatgacaa gaaaaagaaa aaagaaaaaa aactgtcacc gttacaaaaa actgtcacca
5341 ttataagtgt tattatactt ataaaaatca gcaagaacaa caaattttgg tcatcataag
5401 aattcaaaat tacaaatata tcgtaacata aaacattata gtggtcattc catcaatttt
5461 gtttatcgtt taacaaatct gcgatcaaac gatatatttt agtaatataa tttcatctat
5521 cacaacataa ccaattatgt cataactaaa aatactatat tttaaaaaca aatatgttgt
5581 tacatgaaaa tattttggta acattataaa aatctgtcat aagatataac aaatctgcta
5641 tcattagagg tcattatatt tattagtttt ttgtacataa atttcttata acatgataga
5701 tgtttttaga aaattataaa tctaccgagt cattcaaaat aaaacagcca gacactacag
5761 taagtctgtt ataaactatg aaagtatgta gttgaataaa aaaatctgtc aaaaaaataa
5821 atatgtcaaa aaaataaatc agctacaatt gttttagtct attgtaacca tattctaaaa
5881 aatctgtcaa ccaaaaaagt ctgccaaaag aaatatttac ggacaaatag atctgtagta
5941 tataaattag atattaaact tttgtaaaat tattgctaga attaatttga aaggacattt
6001 ttctatttat ttttttacac atgagacaag tgtattttag acatgaaaat aatttaaccc
6061 aaattgtttt taaaataatc tagtaattaa actgataatt tatgttaatc tagtaacttt
6121 cccattatta taatgataaa ttgttgagaa aatttacttt attttagtgt tactttgttc
6181 tagagtaaaa tcgaagaata cttagggagg gacatagggt cagtgccggc ttaacatgga
6241 gaggagggag tgcaacaacg agtggcccat aaagcaaaag ggacacatac aattctttcc
6301 ttttgtaaac ataaaaagaa aaagaaaaaa atatgtgcaa atataacaga aaattgcaaa
6361 tttttactaa aaagctcata tatatttgca ttccttttaa atataaggta aaaaaaaatt
6421 ctaaatttat tgggcccaaa ttatatgttg atatatttta atccaacaac tctaatacaa
6481 atattgattt attgggccaa atctcttttg taaaatgaga ttgttgtttt attttatttt
6541 aaatgatgtt tactaagtgt tttacttttg tacgaaaaca atttccaata tattaagggt
6601 ccaatttttt ttttgcccta ggacctagaa taagcttgag ccggcactgc agagggtcct
6661 aggatttttg tattttttac tttgtaagtg aatgccatag caagccaagg actgaagaag
6721 aacaacggaa aatatagaga atatgaattt gtgtgatgcg aagacgaaaa gaaattatag
6781 aaaatcaact gaaattgatg acgtcgaatt tgattgagga gaaaaaatag ctacaaaata
6841 agaggaagaa aaaggaaaat aaagaaaaga acgaagagaa tgagaaggag aagccgaaaa
6901 gggtctagag aagaggggtg ttttttcatg aagacaagat actacgaaag aaggaagatg
6961 aagggctagt tataagtaga tgtagatctc tgaatatata tatatatata tatatataaa
7021 gtgtctccct aaaccggaaa aaacgatggg ttttgatatg ctgaacgggg gacaaaaccc
7081 gagaaccgaa atcaatttaa aagttcaccg atcactatac aactctgcac ttattgaaac
7141 cgaaaaccaa aattctccgt ttagacggtt ttggttcaat acggttctta ttgaacgtca
7201 atcactctat ctactactat acttgaaaaa caaaattaaa ttttttgcct tgacttgatc
7261 cagagagatg ttacgcggcg gcgatgcatt aatcgataaa atcaaacaac atgccacgca
7321 ttgacttttg caagaagcca gcaactttat ataaaacaat cgatgataat tgtgatcgtt
7381 acaactatgt aataatgcat atatataagc tatgtattaa ctgtaaactt gatttaaata
7441 atactgatgg ggagtatata ccttgatgca tagaataaaa atgtaaaata cgtatgaatt
7501 actagcttag tggtagcctt gttacttgga ttagtttagc aagcaaatat ctagctctaa
7561 ttcacaagct aaccctaaaa gttggttgga catatacatc gtcaaatata tcgaagctat
7621 atatatatat ttaacatatt tgtttttgtt acattaaaac aaacaaaaca aaagaaaaaa
7681 gttttttttg tgctcctcct ttttaatatc ttttatgaaa atggctgact gttatggaaa
7741 atgatgtttt tattaatcta agtagtgcct ttcaccaaag aaaaaaaaaa accaaatata
7801 gtagacttta gtacggagat acgtgaacat aacttgttag attggttttc aacaaaagag
7861 tatagaatgg aaaatcgtta catactgact ctcatgaaca aacatatttt gcttaatgaa
7921 aagtcaactc gatctttatt atttatgagg ataaaaatca aataggctct gtcgacaaaa
7981 cgaaacaatc agactaccct ttagaatata tttaaaatta ccaaaaatat taatctttta
8041 ggaaattaca catgatgata ttttagcata tggcggatat ttacctccaa ctccaaatga
8101 ggatatttta gcatatggcg gtctttattc gtacgtacta ttttgcaaat cattttatat
8161 gattgtatat accatagatt ataatcacta ctcttagtcc cataatttgt aaataatagt
8221 aattcgtaga catagatgat caaagttctt tgtaatctta ctacatacac aaaaagtaat
8281 aaaacacaac atttttgttg tattaaatgt gcttgtccgt tgacaaaaaa gataaaagaa
8341 tagctttgtt tgtcctttct cgacaagttt gtattataat cgtctcacgt tcatttcaag
8401 attacatgca gaatttgtca aatgaagtct aaatcatcaa aagtaatttt tccttatttg
8461 ttctcacttg aatataaatt aacgaaatat gattcaccat aaattacacg aactatagga
8521 tacgggtact tatttattcc cttgcggtaa tacgaatagg tccaagattg gaaatctcag
8581 agttgtcatg aatactaagt taaaatatgt gcagaccata caaaacatag acgagcatag
8641 aaagagaagt tgatcaagtg atggaaaaag attaaaataa aatataaata tggatatacc
8701 cttaagattc cggagactat gcttctccga tctcgtctat aaatttcagc cgtctcagcc
8761 aacacaatca caaccaccac ctccctccct ctctctctct taatctttct gcccccacca
8821 ttagcgcaca acggtgagat tcgttagatg tttattatct atgcatccaa attcacatat
8881 agatagatag tacatatata gttgtttata tcaatagaaa cttttgtttc taaattgtaa
8941 tcgcttaaat tatttgattt atgtataggt ttaattataa atgttattct gttggttgta
9001 ttggtactaa actaaaataa tttagtagca tctttccaat attattataa aattatagtt
9061 aagatattta tttttacttc atgaagcagg aaagacaaga gaggcttaga tggctgaaca
9121 acaactagga gtgctaaagg cactcgatgt tgcgaagacg caactttatc atttcacggc
9181 gattgtcatc gccggtatgg gtttctttac cgatgcgtac gatctttttt gcgtgtcctt
9241 ggtaacaaag ctccttggcc gcatctacta cttcaatccg gagtcagcga agcctggctc
9301 acttccccct catgttgcgg ccgctgtcaa tggtgtggcc ctttgtggaa ccctttctgg
9361 tcaactcttc ttcggttggc tcggtgacaa actcggacgg aaaaaagtgt acggtcttac
9421 tttgatcatg atgatcttat gctctgtcgc ttctggcctc tcttttggca acgaagccaa
9481 gggtgtcatg accacccttt gcttcttcag gtacagtttt catccaatta caatattata
9541 tacacacatg attaaccaat aatctaataa cgaatgcaag ttttaaaagt tagtcacgct
9601 tcgaactgat ttaggtattt ttctttgaga aagttttttt atatataaac cattacgtag
9661 gttttggttg ggatttggta ttggaggtga ctacccactt tctgccacca tcatgtctga
9721 atacgcaaac aagaagaccc gtggggcttt catcgcagct gtcttcgcca tgcaaggtgt
9781 cggtatcttg gctggaggtt tcgtggcact cgcagtatct tctatattcg acaaaaagtt
9841 cccagctcca acatatgcag taaacagggc cctctcaacg cctcctcaag ttgattacat
9901 ttggcgaatc atcgtcatgt ttggtgcttt acccgcagct ttgacttact actggcgtat
9961 gaagatgcct gaaactgccc gttacaccgc tttggttgcc aagaacatca aacaagccac
10021 agccgacatg tccaaggtct tacaaacaga tatcgagctt gaggaaaggg tggaggatga
10081 cgtcaaagac cccagacaaa actatggctt gttctccaag gaattcctta gacgccatgg
10141 acttcatctc cttggaacta cctccacttg gtttttgctt gacattgcct tctacagcca
10201 aaacttgttc cagaaggata ttttctcggc catcggatgg atcccaaagg cagccaccat
10261 gaacgccacc catgaggttt tcaggattgc tagggctcag actcttatcg ccctttgcag
10321 tacagtccca ggctactggt tcacagttgc gtttattgat accattggaa ggtttaagat
10381 ccaactaaat ggatttttca tgatgaccgt gtttatgttt gccattgcct tcccttacaa
10441 ccactggatc aaaccagaaa accgtatcgg atttgtggtt atgtactctc ttactttctt
10501 cttcgccaat tttggtccaa atgcaaccac ttttattgtc cctgctgaga tattcccggc
10561 caggctaagg tcaacatgtc atggaatatc agccgcggct ggtaaggctg gagccatcat
10621 tggagccttc gggttcttat atgcggctca aaatcaagac aaggctaagg tggatgcagg
10681 atacccacca ggtatcggag ttaagaactc attgatcgtg cttggtgttc ttaacttcat
10741 cggtatgctc ttcaccttcc ttgtcccaga gcccaagggc aagtccctcg aagaactctc
10801 tggtgaagct gaagttagcc atgacgagaa ataatttact ttgtgatcaa atgtggagtt
10861 gtttgtggtt tgtttgattt ttttgtttct ttccaaattt tttttttcct ttccttgatg
10921 cagtaagaaa tgtttgattg tgtggaatac ttcagtctta ctgccgtgag tgcaaatctt
10981 gtaatccact tttttgtgtt ctaattaaca ttgtcaaata aagtttctta aactttgatt
11041 ctaactttcc aaacactaac gaactctctg ttgtgcgttt atgtttatca atttgaagca
11101 tgaaaatttg attgttcata tgtttgctat atactgagaa acaccaaaga atagttttta
11161 tggatttcga ttatctgatc aacacattag ttggttatgg tgttcaatgt tcagtgtctt
11221 gtcatcacta acgttatctc tttgccttat atcgacacat tcaagtgggc gtagttgtag
11281 accattagtc ttgtgctctg tttgtaaata acgctattgg ttagttagct ttctttttgg
11341 ttggtgtata tattcttttt gggttgaagc tctgtgtagc gtttagaaat gttaaaagtt
11401 cgatcccaaa atgtagcaag atttgaaatt tctttttgga ttgatgtaat gtatttaact
11461 ggcttcttat gaatcacggt gcttattgtt tgattaatgt gaactgaatt gttctgtgct
11521 tgagattcaa aaagctcaag aggatctaat actaaatagt ctcactaatg catatgtttg
11581 atttgtttca aatattgatt gttctctctg acgcatttgt ttgattattg tgcactacct
11641 gtttgggatg ggcattctat tggtctcact ataccaaatt accaactttg tgaaactcac
11701 cataacttgt gtgatccata aaatcttaga ttaattactg ttaagtttca tgaaatttta
11761 actcaaaaca atttggcaat aaatagattg acattgtatg tccaaaaacc aaactgagta
11821 tttaacaaaa ccaactattt ccgaccaatg aatgcgtcta aaatgtttgt ttccaacaaa
11881 tttctgatca ttgatttcgt cggaactaac acaaataata attcgcataa tcataataat
11941 tctcctaatc agttcataaa cttgaatgac aagttaattt actcatttta ccatgaccat
12001 taattcataa attgagtcac aaattaatat caagatttga ccatgtccct tatgcaagag
12061 ggggttggaa agcgatgggt ctatagtgtt acaattaatt ctctagtatt catataacca
12121 aaacacaatt ataatatttt cacattaaag ataaattaaa aacatccata taatgtttca
12181 catttaataa tatacatatt ttaaatattt tgttaacatt taaaattatg atttttctat
12241 catttttttt attttttaaa ctactttgaa tcatcatata atacataaca ctaattaaaa
12301 tctaattttt tctggatata ttgtgattcg gatgtttgaa aacagccata tattactcac
12361 ataatgaaat aaaattattt gtgttcaaag tctcacatca gctaaaactt tgtgtccaaa
12421 atctcataaa taagaccaaa ttgaacccaa agaaatatga gtgagtagga atcctaactt
12481 aacaaacatc caaatttaaa tatttgttca gtttaattcg gttctttagt caaaccgttt
12541 aattatacca gatatcataa attctaaact ttttaaaaaa ttaaaatatt ataattgatc
12601 tatttaaagt gtataaaaac ttcaaaatat tatcaacgtt taacttataa aaagtttaaa
12661 atatcacggt atgggttata tggtttgacg agtttgaatc ttcattaata taaatttaaa
12721 tatcattaac tcggtggtac aacatgggta agtcatcatt ttaatatttt gttaacacta
12781 gcaatattat aatgatatgt caagttaaat tgattcaatt atgattatgt attaaaacaa
12841 gatatataag tattagtttt ctaggtatct gcaatattag aaaccagata tccaaaagtc
12901 acggatctgg attgttaaag cacagttctg aatacttttc aaattctagg atatccggat
12961 ccattctacc aattacaatc ctgcagaagc cttgttggcc acacaatcga cacaagagtt
13021 agctagccaa aaagtgcctt caacttaaga agatgcaaac cggaaagaaa aaaacatatc
13081 tttggtccga aggaaatcat gttttattat caaatttaac caccaacaac tacaaaccaa
13141 atttacaaca acaacaacca aaaataaaaa cacatacaca caagtgatag cagcagacaa
13201 cacaaaacat aacaaaaaca gaagcttccc aaaattccga aattcaaaat cctctttaat
13261 ttctcatcat atagtagtcg agttacgttg gcatggtttc taatagttat tatgcggagt
13321 tttaaatctt catcagttgt attctttcga attttgcgaa ctcagaatcg actgcaaata
13381 aacatgttta attagatcaa atggttagag attccgagct tgccttgtaa catgacatga
13441 caacaatgat ttggctataa attacttgta agttactagt gagaaacata gcaaaccaat
13501 tttcaagtat accacagtgt tagttttgac taacattagg tctaattcgg tgaagtcaat
13561 actaatccaa agtttcatct atcaatgcga atagatttaa aattattgag gagcacaaac
13621 agattcaaaa tgttaaatca ttcaaaaaca tttagtaata gagcaaatta ttatgcattt
13681 accatatttc attttttgtg ctacagtata tccacaaatt aaaaagtgat gtgattaata
13741 cataccttca ctttaggata agggaagcta gttttagcac cacctctgct tccgaaactg
13801 aactttgatt tcttgtcatt agattttaag atctgaaaag agcaggtcaa atcctcagac
13861 acactcatca tcgcacctgc attgtcaaat tccccacagt aattcggcgc agagaatatc
13921 gttacgagct gcttattcgc aaagaactcg aatccatctt caacaacctg ttacaaaatc
13981 gttgcttgtg tcaaaaggaa acacgttaaa aatgtttctt tgggcacaag gaacaataaa
14041 caaacctggt gagccctaca aatgaggtca agatcgagtc ttttaagaaa tccagaaact
14101 atatctgatc caaaagtgta agaaactccg cgatcgttag gcccccaacc tctaacatct
14161 ttatcaggat cagaccacaa gagatcacag agtaaaccac gatcaggaat atccgttgga
14221 cgacgaatat ccctaatctg cctcaagctt agcagctccg gggagagccc accatgcata
14281 caaaaaatcc gctcatcgat gagtgcagcg acggggagac agttgaagca atcagtgaag
14341 attcgccaaa tcttgacact gaatctacgt ttacactcgt catagaagcc gtaaatacga
14401 ttgattgatg cactttcatg gtttcctctg agaaggaaga agttttcagg gaacttaatc
14461 ttgtaagcaa gtaaaagaca aatcgtttcg aggctttgct tgccgcgatc gacataatct
14521 ccaagaaaca agtagtttga attaggaggg tatccgccat gttcgaatag tctcaagaga
14581 tccggatatt gtccatgaat gtcccctgtt tattatttgt ttaaccaaaa gattacatag
14641 gagcacaatc tcagaaaaga gtcagagaga aacagagaga aacgtaccac atattttaac
14701 aggagcttca agttccaaga gatttggttg tctcaagaag atatctctag agacgaaaca
14761 gagctgtttg atctctgttt cagacaactg aacaatcttt cctggttttt ctctagcttc
14821 aagcaaccta ttgatcaccg agtttaaagt accaggatcc atttataaaa cctcaagaaa
14881 cagagcaaca atgagacaaa acaacaacaa cgcctagaag ctttataata tacaaaagct
14941 attgacaata tggaataaat ttaaaaagta tggagctttt atgttctttt atgcttgctc
15001 tttgagaaaa agaatggtga ttgtcaaatt caagaagggc tttataaagc cgcgtttaag
15061 aatatattct tcataccttt ttagcaaaag tcacatgtgt tcatacctac ctacattgtt
15121 ctaacttcaa ctaaatcata taataatcag attctagcta atttagtaac acattgtttg
15181 tgtgtgtgtt aagcttcaca ggattgaatc ctgtccggta cgtcatgatt caaatttaag
15241 atctttccgc cggtactttg agtgcgaaaa acgctctttc ttcttcttct agtatcgatg
15301 tttttggatt cgtccgcgtc atattaagca acttggctat ttacaaaaaa catattcttc
15361 ttcctttgta gccaaaagcc atgggtaaaa acctacccta attgttctaa cttcaactac
15421 atctaataac caaaaatgtt agctatattt tctcttttat ggtcaaaaca tattagctag
15481 tttagacact ttctttgtgt tgagcttttt aggcttgaat ctaatcgaat tc
15541
//
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