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<FONT FACE="Verdana"><SPAN STYLE='font-size:12.0px'>I am using eprimer3 and multiple fasta formatted dinucleotide containing sequences as input. I find that eprimer3/primer3_core will handle an input file containing up to 23 sequences; at 24 sequences, both eprimer3 and primer3_core “sleep”. i.e. the processes never acheive run status when I watch what happens using top or prstat. <BR>
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I don’t know if this has something to do with limitations of my system (sun ultra5/1GB ram/500 MB free disk space) or intrinsic to eprimer3/primer3_core. I do know that it also happens on a Mac OS X system (640 MB ram/35 GB free disk space).<BR>
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Is there a config file that sets limits on eprimer3? (that i can change?)<BR>
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Iain Drummond, Ph.D.<BR>
Assistant Professor<BR>
Department of Medicine, Harvard Medical School and <BR>
Renal Unit, Massachusetts General Hospital <BR>
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Mailing address:<BR>
Renal Unit / MGH 149-8000<BR>
149 13th St. <BR>
Charlestown, MA 02129<BR>
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Tel: 617 726 5647<BR>
Fax: 617 726 5669<BR>
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idrummond@partners.org<BR>
idrummon@receptor.mgh.harvard.edu<BR>
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Lab Home Page:<BR>
<a href="http://danio.mgh.harvard.edu">http://danio.mgh.harvard.edu</a><BR>
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