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<div class=Section1>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>Hi all,<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'><o:p> </o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>I have recently installed emboss version 2.6.<span
style='mso-spacerun:yes'> </span>When my one of my users ran <span
class=SpellE>estgenome</span>, she got the following error messages.<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'><o:p> </o:p></span></font></p>
<p class=MsoNormal style='mso-layout-grid-align:none;text-autospace:none'><font
size=2 face="Courier New"><span style='font-size:10.0pt;font-family:"Courier New"'>~>
est2genome<o:p></o:p></span></font></p>
<p class=MsoNormal style='mso-layout-grid-align:none;text-autospace:none'><font
size=2 face="Courier New"><span style='font-size:10.0pt;font-family:"Courier New"'>Align
EST and genomic DNA sequences<o:p></o:p></span></font></p>
<p class=MsoNormal style='mso-layout-grid-align:none;text-autospace:none'><font
size=2 face="Courier New"><span style='font-size:10.0pt;font-family:"Courier New"'>EST
sequence(s): park2musdowngene.txt<o:p></o:p></span></font></p>
<p class=MsoNormal style='mso-layout-grid-align:none;text-autospace:none'><font
size=2 face="Courier New"><span style='font-size:10.0pt;font-family:"Courier New"'>Genomic
sequence: park2mousecontig.txt<o:p></o:p></span></font></p>
<p class=MsoNormal style='mso-layout-grid-align:none;text-autospace:none'><span
class=GramE><font size=2 face="Courier New"><span style='font-size:10.0pt;
font-family:"Courier New"'>Output file</span></font></span><font size=2
face="Courier New"><span style='font-size:10.0pt;font-family:"Courier New"'>
[mus.est2genome]: hope7.estgenome<o:p></o:p></span></font></p>
<p class=MsoNormal style='mso-layout-grid-align:none;text-autospace:none'><font
size=2 face="Courier New"><span style='font-size:10.0pt;font-family:"Courier New"'>Error:
<span class=SpellE>rpairs</span> 2500001 beyond maximum 2500000<o:p></o:p></span></font></p>
<p class=MsoNormal style='mso-layout-grid-align:none;text-autospace:none'><font
size=2 face="Courier New"><span style='font-size:10.0pt;font-family:"Courier New"'>Error:
increase space threshold to repeat this search<o:p></o:p></span></font></p>
<p class=MsoNormal style='mso-layout-grid-align:none;text-autospace:none'><font
size=2 face="Courier New"><span style='font-size:10.0pt;font-family:"Courier New"'>Error:
stack ran out of memory, returning NULL<o:p></o:p></span></font></p>
<p class=MsoNormal style='mso-layout-grid-align:none;text-autospace:none'><font
size=2 face="Courier New"><span style='font-size:10.0pt;font-family:"Courier New"'>Error:
<span class=SpellE>rpairs</span> 2500001 beyond maximum 2500000<o:p></o:p></span></font></p>
<p class=MsoNormal style='mso-layout-grid-align:none;text-autospace:none'><font
size=2 face="Courier New"><span style='font-size:10.0pt;font-family:"Courier New"'>………<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'><o:p> </o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>Is there a configuration file to tweak so as to increase the
stack?<span style='mso-spacerun:yes'> </span>Do I have to recompile
emboss with some special settings?<span style='mso-spacerun:yes'>
</span>Any help would be most appreciated.<span style='mso-spacerun:yes'>
</span>Thanks.<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'><o:p> </o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>Regards,<o:p></o:p></span></font></p>
<p class=MsoNormal style='mso-layout-grid-align:none'><st1:PersonName><font
size=2 face="Courier New"><span style='font-size:10.0pt;font-family:"Courier New";
mso-no-proof:yes'>Chan Chee Seng</span></font></st1:PersonName><span
style='mso-no-proof:yes'><o:p></o:p></span></p>
<p class=MsoNormal style='mso-layout-grid-align:none'><font size=2
face="Courier New"><span style='font-size:10.0pt;font-family:"Courier New";
mso-no-proof:yes'>UNIX administrator<o:p></o:p></span></font></p>
<p class=MsoNormal style='mso-layout-grid-align:none'><font size=2
face="Courier New"><span style='font-size:10.0pt;font-family:"Courier New";
mso-no-proof:yes'>Genome Institute of </span></font><st1:country-region><st1:place><font
size=2 face="Courier New"><span style='font-size:10.0pt;font-family:"Courier New";
mso-no-proof:yes'><st1:country-region><st1:place>Singapore</span></font></st1:place></st1:country-region></st1:place></st1:country-region><font
size=2 face="Courier New"><span style='font-size:10.0pt;font-family:"Courier New";
mso-no-proof:yes'><o:p></o:p></span></font></p>
<p class=MsoNormal style='mso-layout-grid-align:none'><st1:Street><st1:address><st1:Street><st1:address><font
size=2 face="Courier New"><span style='font-size:10.0pt;font-family:"Courier New";
mso-no-proof:yes'>1 Science Park Road</span></font></st1:address></st1:Street></st1:address></st1:Street><font
size=2 face="Courier New"><span style='font-size:10.0pt;font-family:"Courier New";
mso-no-proof:yes'><o:p></o:p></span></font></p>
<p class=MsoNormal style='mso-layout-grid-align:none'><font size=2
face="Courier New"><span style='font-size:10.0pt;font-family:"Courier New";
mso-no-proof:yes'>The Capricorn #05-01<o:p></o:p></span></font></p>
<p class=MsoNormal style='mso-layout-grid-align:none'><st1:country-region><st1:place><st1:country-region><st1:place><font
size=2 face="Courier New"><span style='font-size:10.0pt;font-family:"Courier New";
mso-no-proof:yes'>Singapore</span></font></st1:place></st1:country-region></st1:place></st1:country-region><font
size=2 face="Courier New"><span style='font-size:10.0pt;font-family:"Courier New";
mso-no-proof:yes'> 117528<o:p></o:p></span></font></p>
<p class=MsoNormal style='mso-layout-grid-align:none'><font size=2
face="Courier New"><span style='font-size:10.0pt;font-family:"Courier New";
mso-no-proof:yes'>Web : <a href="http://www.gis.a-star.edu.sg/">http://www.gis.a-star.edu.sg</a></span></font><font
size=2 face="Courier New"><span style='font-size:10.0pt;font-family:"Courier New"'><o:p></o:p></span></font></p>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'><o:p> </o:p></span></font></p>
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