From ahmet.durak at ego.com.tr Tue Nov 6 01:42:51 2012 From: ahmet.durak at ego.com.tr (ahmet durak) Date: Tue, 6 Nov 2012 08:42:51 +0200 Subject: [EMBOSS] Material Safety Data Sheet Requery Message-ID: <4801E12C06505646B7E4EB9E86202156044665F0@egotr04.local.ego.com.tr> Hi; I need a Material Safety Data Sheet of Metanol. Can you help me to find it's MSDS? ?f you send it to my email I will be grateful. My best regards. Ahmet Durak E.G.O. Elektrikli Aletler San.A.?. Sat?nalma Uzman? / Purchasing Specialist E5 Karayolu ?zeri , Misinli Kav?a?? 59851 ?orlu , Tekirda?, Turkey Phone:+90 282 684 68 00 Fax:+90 282 684 68 70 ahmet.durak at ego.com.tr http://www.egoproducts.com This message has been scanned by Trend Micro%CRLF%IMSS against virus%CRLF% From d.m.a.martin at dundee.ac.uk Tue Nov 20 05:44:45 2012 From: d.m.a.martin at dundee.ac.uk (David Martin) Date: Tue, 20 Nov 2012 10:44:45 +0000 Subject: [EMBOSS] Issues with digest In-Reply-To: <959CFF5060375249824CC633DDDF896F1B6F21F8@AMSPRD0410MB350.eurprd04.prod.outlook.com> References: <959CFF5060375249824CC633DDDF896F1B6F21F8@AMSPRD0410MB350.eurprd04.prod.outlook.com> Message-ID: <959CFF5060375249824CC633DDDF896F1B6F2248@AMSPRD0410MB350.eurprd04.prod.outlook.com> We have run across some issues with digest. When trying to digest the following peptide we do not get all the expected fragments. As you can see the fragments 10-31 and 16-31 is missing as are some others. Is this peculiar to this sequence or is there an issue in the application? ..d $ digest asis::ACDEFGHIKLMNPQRSTVWYKKACDPRFGHI -over -un -all -stdout Protein proteolytic enzyme or reagent cleavage digest Enzymes and Reagents 1 : Trypsin 2 : Lys-C 3 : Arg-C 4 : Asp-N 5 : V8-bicarb 6 : V8-phosph 7 : Chymotrypsin 8 : CNBr Select number [1]: Output report [stdout]: ######################################## # Program: digest # Rundate: Tue 20 Nov 2012 10:40:26 # Commandline: digest # [-seqall] asis::ACDEFGHIKLMNPQRSTVWYKKACDPRFGHI # -overlap # -unfavoured # -allpartials # -stdout # Report_format: seqtable # Report_file: stdout ######################################## #======================================= # # Sequence: asis from: 1 to: 31 # HitCount: 6 # # Complete digestion with Trypsin yields 6 fragments # #======================================= Start End Mol_Weight Cterm Nterm Sequence 1 9 1019.133 . L ACDEFGHIK 16 21 782.888 R K STVWYK 10 15 757.900 K S LMNPQR 23 27 560.620 K F ACDPR 28 31 472.538 R . FGHI 22 22 146.184 K A K #--------------------------------------- #--------------------------------------- #======================================= # # Sequence: asis from: 1 to: 31 # HitCount: 9 # # # # Partial digest with Trypsin yields 9 extras. # All partials shown: # #======================================= Start End Mol_Weight Cterm Nterm Sequence 1 27 3194.681 . F ACDEFGHIKLMNPQRSTVWYKKACDPR 1 22 2652.072 . A ACDEFGHIKLMNPQRSTVWYKK 1 21 2523.899 . K ACDEFGHIKLMNPQRSTVWYK 10 27 2193.559 K F LMNPQRSTVWYKKACDPR 1 15 1759.022 . S ACDEFGHIKLMNPQR 10 22 1650.950 K A LMNPQRSTVWYKK 10 21 1522.777 K K LMNPQRSTVWYK 16 27 1453.670 R F STVWYKKACDPR 16 22 911.061 R A STVWYKK #--------------------------------------- #--------------------------------------- -bash-3.2$ The University of Dundee is a registered Scottish Charity, No: SC015096 From a.bruselles at gmail.com Tue Nov 20 06:05:01 2012 From: a.bruselles at gmail.com (Alessandro Bruselles) Date: Tue, 20 Nov 2012 12:05:01 +0100 Subject: [EMBOSS] eprimer3 error Message-ID: Hi, when I try using eprimer3 I receive the following error: Error: Missing SEQUENCE tag any idea? Thanks ___________________________ From ahmet.durak at ego.com.tr Tue Nov 6 06:42:51 2012 From: ahmet.durak at ego.com.tr (ahmet durak) Date: Tue, 6 Nov 2012 08:42:51 +0200 Subject: [EMBOSS] Material Safety Data Sheet Requery Message-ID: <4801E12C06505646B7E4EB9E86202156044665F0@egotr04.local.ego.com.tr> Hi; I need a Material Safety Data Sheet of Metanol. Can you help me to find it's MSDS? ?f you send it to my email I will be grateful. My best regards. Ahmet Durak E.G.O. Elektrikli Aletler San.A.?. Sat?nalma Uzman? / Purchasing Specialist E5 Karayolu ?zeri , Misinli Kav?a?? 59851 ?orlu , Tekirda?, Turkey Phone:+90 282 684 68 00 Fax:+90 282 684 68 70 ahmet.durak at ego.com.tr http://www.egoproducts.com This message has been scanned by Trend Micro%CRLF%IMSS against virus%CRLF% From d.m.a.martin at dundee.ac.uk Tue Nov 20 10:44:45 2012 From: d.m.a.martin at dundee.ac.uk (David Martin) Date: Tue, 20 Nov 2012 10:44:45 +0000 Subject: [EMBOSS] Issues with digest In-Reply-To: <959CFF5060375249824CC633DDDF896F1B6F21F8@AMSPRD0410MB350.eurprd04.prod.outlook.com> References: <959CFF5060375249824CC633DDDF896F1B6F21F8@AMSPRD0410MB350.eurprd04.prod.outlook.com> Message-ID: <959CFF5060375249824CC633DDDF896F1B6F2248@AMSPRD0410MB350.eurprd04.prod.outlook.com> We have run across some issues with digest. When trying to digest the following peptide we do not get all the expected fragments. As you can see the fragments 10-31 and 16-31 is missing as are some others. Is this peculiar to this sequence or is there an issue in the application? ..d $ digest asis::ACDEFGHIKLMNPQRSTVWYKKACDPRFGHI -over -un -all -stdout Protein proteolytic enzyme or reagent cleavage digest Enzymes and Reagents 1 : Trypsin 2 : Lys-C 3 : Arg-C 4 : Asp-N 5 : V8-bicarb 6 : V8-phosph 7 : Chymotrypsin 8 : CNBr Select number [1]: Output report [stdout]: ######################################## # Program: digest # Rundate: Tue 20 Nov 2012 10:40:26 # Commandline: digest # [-seqall] asis::ACDEFGHIKLMNPQRSTVWYKKACDPRFGHI # -overlap # -unfavoured # -allpartials # -stdout # Report_format: seqtable # Report_file: stdout ######################################## #======================================= # # Sequence: asis from: 1 to: 31 # HitCount: 6 # # Complete digestion with Trypsin yields 6 fragments # #======================================= Start End Mol_Weight Cterm Nterm Sequence 1 9 1019.133 . L ACDEFGHIK 16 21 782.888 R K STVWYK 10 15 757.900 K S LMNPQR 23 27 560.620 K F ACDPR 28 31 472.538 R . FGHI 22 22 146.184 K A K #--------------------------------------- #--------------------------------------- #======================================= # # Sequence: asis from: 1 to: 31 # HitCount: 9 # # # # Partial digest with Trypsin yields 9 extras. # All partials shown: # #======================================= Start End Mol_Weight Cterm Nterm Sequence 1 27 3194.681 . F ACDEFGHIKLMNPQRSTVWYKKACDPR 1 22 2652.072 . A ACDEFGHIKLMNPQRSTVWYKK 1 21 2523.899 . K ACDEFGHIKLMNPQRSTVWYK 10 27 2193.559 K F LMNPQRSTVWYKKACDPR 1 15 1759.022 . S ACDEFGHIKLMNPQR 10 22 1650.950 K A LMNPQRSTVWYKK 10 21 1522.777 K K LMNPQRSTVWYK 16 27 1453.670 R F STVWYKKACDPR 16 22 911.061 R A STVWYKK #--------------------------------------- #--------------------------------------- -bash-3.2$ The University of Dundee is a registered Scottish Charity, No: SC015096 From a.bruselles at gmail.com Tue Nov 20 11:05:01 2012 From: a.bruselles at gmail.com (Alessandro Bruselles) Date: Tue, 20 Nov 2012 12:05:01 +0100 Subject: [EMBOSS] eprimer3 error Message-ID: Hi, when I try using eprimer3 I receive the following error: Error: Missing SEQUENCE tag any idea? Thanks ___________________________