[emboss-dev] EMBOSS seqret FASTQ support

Peter Rice pmr at ebi.ac.uk
Thu Jul 23 12:24:01 EDT 2009

Peter wrote:
> I've got another issue for you, which I think is an rounding problem
> converting negative Solexa scores into ASCII (which sounds a bit
> strange), or assuming you store everything as PHRED scores in
> memory, this could be in how you round negative Solexa scores
> on conversion back to ASCII.

Yup, it's the rounding on output. It was adding 0.5 and going to the 
nearest integer.

For negative values of course it has to subtract 0.5 to get the correct 



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