[Biopython] Qblast : no hits

Chris Fields cjfields at illinois.edu
Fri Apr 23 12:00:07 UTC 2010


On Apr 23, 2010, at 4:49 AM, Peter wrote:

>> ...
> 
> Differences between a manual BLAST search on the NCBI website
> and a script search via QBLAST are almost always down to different
> parameter settings. The NCBI have often adjusted the defaults on
> the website, and they no longer match the defaults on QBLAST.
> You should check things like the expectation cut off, the matrix,
> gap penalties etc. The simplest option would be just to copy the
> current defaults from the website into your python code.
> 
> We probably need to put this into the Biopython FAQ ...
> 
> Regards,
> 
> Peter

Same for BioPerl.

chris



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