Ah, I should add this is a BLASTX. On Fri, 2008-11-14 at 14:56 +0000, Alexie Papanicolaou wrote: > Dear all, > > Would anyone have an insight into this error while parsing a BLAST > report (bioperl from CVS on 28th of August)? > > ------------- EXCEPTION: Bio::Root::Exception ------------- > MSG: Undefined sub-sequence (548,548). Valid range = 51 - 548 > STACK: Error::throw > STACK: > Bio::Root::Root::throw /usr/local/share/perl/5.8.8/Bio/Root/Root.pm:357 > STACK: > Bio::Search::HSP::HSPI::matches /usr/local/share/perl/5.8.8/Bio/Search/HSP/HSPI.pm:691 > STACK: > Bio::Search::SearchUtils::_adjust_contigs /usr/local/share/perl/5.8.8/Bio/Search/SearchUtils.pm:421 > STACK: > Bio::Search::SearchUtils::tile_hsps /usr/local/share/perl/5.8.8/Bio/Search/SearchUtils.pm:200 > STACK: > Bio::Search::Hit::GenericHit::strand /usr/local/share/perl/5.8.8/Bio/Search/Hit/GenericHit.pm:1455 > > This is using HSPI.pm with the patch starting > ## ML: START fix for substr out of range error > > many thanks for any pointers > a > > Alexie Papanicolaou > Entomology > Max Planck Institute for Chemical Ecology > Hans Knoell Str 8 > Jena 07745 > Germany > Email apapanicolaou@ice.mpg.de > Tel +493641571561 > > > -- -- "Eppur si evolve" ("And yet it evolves") -Galileo Jr (ca 21st century) "One Galileo in two thousand years is enough." -Pope Pius XII -- Alexie Papanicolaou Entomology Max Planck Institute for Chemical Ecology Hans Knoell Str 8 Jena 07745 Germany Email apapanicolaou@ice.mpg.de Tel +493641571561