Dear all, Would anyone have an insight into this error while parsing a BLAST report (bioperl from CVS on 28th of August)? ------------- EXCEPTION: Bio::Root::Exception ------------- MSG: Undefined sub-sequence (548,548). Valid range = 51 - 548 STACK: Error::throw STACK: Bio::Root::Root::throw /usr/local/share/perl/5.8.8/Bio/Root/Root.pm:357 STACK: Bio::Search::HSP::HSPI::matches /usr/local/share/perl/5.8.8/Bio/Search/HSP/HSPI.pm:691 STACK: Bio::Search::SearchUtils::_adjust_contigs /usr/local/share/perl/5.8.8/Bio/Search/SearchUtils.pm:421 STACK: Bio::Search::SearchUtils::tile_hsps /usr/local/share/perl/5.8.8/Bio/Search/SearchUtils.pm:200 STACK: Bio::Search::Hit::GenericHit::strand /usr/local/share/perl/5.8.8/Bio/Search/Hit/GenericHit.pm:1455 This is using HSPI.pm with the patch starting ## ML: START fix for substr out of range error many thanks for any pointers a Alexie Papanicolaou Entomology Max Planck Institute for Chemical Ecology Hans Knoell Str 8 Jena 07745 Germany Email apapanicolaou@ice.mpg.de Tel +493641571561