[Bioperl-l] not recognizing hsp in a hit

pageski Cynthia.Page at Colorado.EDU
Tue Apr 5 17:50:28 UTC 2011


Hi I am new to this forum so please forgive me if I make faux pas!

I am parsing a BLAST report using bioperl for some reason one of my hits
which list three hsp is not recognizing them as hsp and during the parsing
they are getting skipped over. I have print to screen output showing two
genes the first is the one I am referring to and the second is one that is
working just for your comparison. I will also paste part of the BLAST report
for those two entries.

I will not paste the code at this time, but will gladly if it is needed. I
am thinking that perhaps I am misunderstanding what an hsp is ... that is
why I am first showing you these two pieces of informations.

Thanks for any help!
##############################################################
I have pasted screen output from my script I use to trouble shoot plus part
of 
a BLAT output in Blast format. From the BLAST output you can see I have
three hits on Chr3
it seems to me that this should be three hsp's however these are not
considered hsp for some reason
yet YCL068 is - I pasted part of that report below YCL067

############screen output##########################################
YCL067C
C
3
chr3
This is the number of hsps: 0 for YCL067C
Number of hsp == :0 for  YCL067C
This is the hitscore: 1222 for name: YCL067C 
 This is the highesthit score: 1222 for Name: YCL067C
YCL067C


YCL068C
C
3
chr3
This is the number of hsps: 3 for YCL068C
This is the number of hsps:3 for name: YCL068C 
 I am in the if hsps >0 and evaluating YCL068C
This is the number of hits:3 for name: YCL068C 
This is the hitscore: 1508 for name: YCL068C 
 This is the highesthit score: 1508 for Name: YCL068C
 This is the percent of hsp percentid:99.6173469387755
######################################################

###################Part of Blast report for YCL067C################


Query= YCL067C
         (633 letters)

Database: /data/genomes/Sigma1278b/sigmav7.fa 
           16 sequences; 11,945,947 total letters

Searching.done
                                                                 Score    E
Sequences producing significant alignments:                      (bits)
Value

chr3                                                                 1222  
0.0
chr3                                                                 1222  
0.0
chr3                                                                 1078  
0.0



>chr3 
          Length = 319572

 Score = 1222 bits (3153), Expect = 0.0
 Identities = 633/633 (100%)
 Strand = Minus / Plus
############################################
Query= YCL068C
         (783 letters)

Database: /data/genomes/Sigma1278b/sigmav7.fa 
           16 sequences; 11,945,947 total letters

Searching.done
                                                                 Score    E
Sequences producing significant alignments:                      (bits)
Value

chr3                                                                 1508  
0.0
chr3                                                                 1505  
0.0
chr3                                                                   91  
1e-18



>chr3 
          Length = 319572

 Score = 1508 bits (3891), Expect = 0.0
 Identities = 781/784 (100%)
 Strand = Minus / Plus

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