[Bioperl-l] can't retrive certain sequences from remote db

Jason Stajich jason at cgt.mc.duke.edu
Tue Feb 11 11:38:14 EST 2003


Do they actually show up if you enter them at the
NCBI search page.  What about from the EMBL search page at EBI?  What
about using the Bio::DB::EMBL interface to search EMBL instead of
searching GenBank?

Are you actually searching a protein accession against a Nucleotide
database or vice-versa?

Various reasons could explain the problems but we'd need to know the
answers to those questions first.

-jason

On Tue, 11 Feb 2003, Alice Lu wrote:

> Hi everyone,
>
> Why is that I can't retrieve certain sequences from remote database? For the following example, I have tried:
>
>  $seq=$gb->get_Seq_by_Acc("AJ322540");
> or
>  $seq=$gb->get_Seq_by_gi("15866774");
>
> Both failed. In the meantime, it works for some other Accession Numbers. it looks like some sequences just aren't in the database even though they have accession number and GI number. The reason? I don't know.
>
> Any suggestions will be appreciated.
>
> Alice
>  
>
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--
Jason Stajich
Duke University
jason at cgt.mc.duke.edu



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